公开(公告)号：US08222385B2

公开(公告)日：2012-07-17

申请号：US12532304

申请日：2008-03-26

申请人： Goro Yoshizaki ,  Yutaka Takeuchi ,  Kazue Nagasawa ,  Kentaro Higuchi ,  Tetsuro Morita ,  Naoki Kabeya

发明人： Goro Yoshizaki ,  Yutaka Takeuchi ,  Kazue Nagasawa ,  Kentaro Higuchi ,  Tetsuro Morita ,  Naoki Kabeya

IPC分类号： C07H21/04 ,  C07H21/02 ,  C12N5/00 ,  C12N5/02 ,  A01K67/027

CPC分类号： C07K14/461 ,  C12Q1/6888 ,  C12Q2600/158

摘要： In order to examine whether or not a germ cell derived from a donor fish, which has been transplanted into a recipient fish of a different species by a surrogate fish technique, grows or matures in the gonad of the recipient fish, it is necessary to use, as an indicator, a trait that is specifically expressed in the germ cell and can be used to distinguish the recipient fish from the donor fish. Vasa gene, which is a germ cell-specific gene, is specific to a primordial germ cell and a spermatogonium/an oogonium, and it is not expressed in a somatic cell. In the present invention, the Vasa gene sequences of a tuna, a chub mackerel, a spotted mackerel, an eastern little tuna, and a drumfish are determined, and the expression of such gene is used as a marker for a germ cell. In addition, according to the present invention, it is possible to specifically detect only a tuna Vasa gene in Vasa gene sequences that are highly conserved in fishes, without sequencing. Thus, a tuna-derived germ cell can be reliably and simply identified in the gonad of the recipient fish. As a result, the growth or breeding of tuna can be carried out with good efficiency. Moreover, utilizing the aforementioned findings, even in a case in which not only a tuna but also another Perciformes fish is used as a donor, a germ cell derived from the donor fish can be efficiently detected from the gonad of a recipient fish of a different species.

摘要翻译： 为了检查是否通过代孕鱼技术移植到不同物种的受体鱼中的源自供体鱼的生殖细胞在受体鱼的性腺中生长或成熟，则必须使用 作为指标，是在生殖细胞中特异性表达并可用于将受体鱼与供体鱼区分开的特征。 Vasa基因是生殖细胞特异性基因，对原始生殖细胞和精原细胞/卵母细胞是特异性的，并且它不在体细胞中表达。 在本发明中，确定了金枪鱼，鲭鱼，斑点m鱼，东部小金枪鱼和鼓鱼的Vasa基因序列，并将该基因的表达用作生殖细胞的标记物。 此外，根据本发明，可以仅在鱼中高度保守的Vasa基因序列中仅特异性检测金枪鱼Vasa基因，而不进行测序。 因此，可以在受体鱼的性腺中可靠且简单地鉴定金枪鱼衍生的生殖细胞。 结果，金枪鱼的生长或繁殖可以以良好的效率进行。 此外，利用上述发现，即使不仅使用金枪鱼，而且使用另一种鲈形鱼作为供体的情况，可以从不同的受体鱼的性腺中有效地检测来自供体鱼的生殖细胞 种类。

公开(公告)号：US20100132056A1

公开(公告)日：2010-05-27

申请号：US12532304

申请日：2008-03-26

申请人： Goro Yoshizaki ,  Yutaka Takeuchi ,  Kazue Nagasawa ,  Kentaro Higuchi ,  Tetsuro Morita ,  Naoki Kabeya

发明人： Goro Yoshizaki ,  Yutaka Takeuchi ,  Kazue Nagasawa ,  Kentaro Higuchi ,  Tetsuro Morita ,  Naoki Kabeya

IPC分类号： A01K67/027 ,  G01N33/50 ,  C07K14/435 ,  C07H21/04 ,  C12N15/63 ,  C07K16/18 ,  C07H21/02 ,  C12Q1/68

CPC分类号： C07K14/461 ,  C12Q1/6888 ,  C12Q2600/158

摘要： In order to examine whether or not a germ cell derived from a donor fish, which has been transplanted into a recipient fish of a different species by a surrogate fish technique, grows or matures in the gonad of the recipient fish, it is necessary to use, as an indicator, a trait that is specifically expressed in the germ cell and can be used to distinguish the recipient fish from the donor fish. Vasa gene, which is a germ cell-specific gene, is specific to a primordial germ cell and a spermatogonium/an oogonium, and it is not expressed in a somatic cell. In the present invention, the Vasa gene sequences of a tuna, a chub mackerel, a spotted mackerel, an eastern little tuna, and a drumfish are determined, and the expression of such gene is used as a marker for a germ cell. In addition, according to the present invention, it is possible to specifically detect only a tuna Vasa gene in Vasa gene sequences that are highly conserved in fishes, without sequencing. Thus, a tuna-derived germ cell can be reliably and simply identified in the gonad of the recipient fish. As a result, the growth or breeding of tuna can be carried out with good efficiency. Moreover, utilizing the aforementioned findings, even in a case in which not only a tuna but also another Perciformes fish is used as a donor, a germ cell derived from the donor fish can be efficiently detected from the gonad of a recipient fish of a different species.

摘要翻译： 为了检查是否通过代孕鱼技术移植到不同物种的受体鱼中的源自供体鱼的生殖细胞在受体鱼的性腺中生长或成熟，则必须使用 作为指标，是在生殖细胞中特异性表达并可用于将受体鱼与供体鱼区分开的特征。 Vasa基因是生殖细胞特异性基因，对原始生殖细胞和精原细胞/卵母细胞是特异性的，并且它不在体细胞中表达。 在本发明中，确定了金枪鱼，鲭鱼，斑点m鱼，东部小金枪鱼和鼓鱼的Vasa基因序列，并将该基因的表达用作生殖细胞的标记物。 此外，根据本发明，可以仅在鱼中高度保守的Vasa基因序列中仅特异性检测金枪鱼Vasa基因，而不进行测序。 因此，可以在受体鱼的性腺中可靠且简单地鉴定金枪鱼衍生的生殖细胞。 结果，金枪鱼的生长或繁殖可以以良好的效率进行。 此外，利用上述发现，即使不仅使用金枪鱼，而且使用另一种鲈形鱼作为供体的情况，可以从不同的受体鱼的性腺中有效地检测来自供体鱼的生殖细胞 种类。