公开(公告)号：US20090307802A1

公开(公告)日：2009-12-10

申请号：US12160725

申请日：2007-01-10

申请人： Greg F.W. Gocal ,  Mark E. Knuth ,  Peter R. Beetham

发明人： Greg F.W. Gocal ,  Mark E. Knuth ,  Peter R. Beetham

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/87 ,  C07K14/415 ,  C12N15/74 ,  C12N1/20

CPC分类号： C12N15/8275 ,  C12N9/1092 ,  C12N15/8213

摘要： The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. Additionally the present invention relates to mutant E. coli cells that contain mutated EPSPS genes.

摘要翻译： 本发明涉及对膦酰甲基甘氨酸家族例如草甘膦的除草剂具有抗性或耐受性的非转基因植物的生产。 本发明还涉及重组型寡核苷酸在编码5-烯醇丙酮酸莽草酸-3-磷酸合成酶（EPSPS）的基因中的植物的染色体或附加型序列中进行所需的突变的用途。 基本上保持野生型蛋白质的催化活性的突变蛋白质允许植物对膦酰基甲基甘氨酸家族的除草剂的抗性或耐受性增加，并允许植物，其器官的基本上正常的生长或发育， 组织或细胞，与野生型植物相比，无论是否存在除草剂。 此外，本发明涉及含有突变的EPSPS基因的突变大肠杆菌细胞。

公开(公告)号：US20170051219A1

公开(公告)日：2017-02-23

申请号：US13752330

申请日：2013-01-28

申请人： Mark Knuth ,  Peter R. Beetham ,  Keith Walker ,  Greg F.W. Gocal

发明人： Mark Knuth ,  Peter R. Beetham ,  Keith Walker ,  Greg F.W. Gocal

IPC分类号： C10L1/02 ,  C11C3/00 ,  C11C1/08 ,  C12N15/82

CPC分类号： C10L1/026 ,  A23D9/00 ,  C10L2200/0476 ,  C11C1/08 ,  C11C3/003 ,  C12N9/0083 ,  C12N15/8247 ,  Y02E50/13

摘要： Provided herein are blends oils or fatty acids comprising more than 50% medium chain fatty acids, or the fatty acid alkyl esters thereof, and having low melting points. Such blends are useful as a fuel or as a starting material for the production of, for example, a biodiesel. Also provided genetically altered or modified plants, modified such that the amount of medium chain fatty acids generated by the plant are increased. Further provided is a method of predicting the melting point of a blend of fatty acid methyl esters and the use of such a method for identifying blends suitable for use as, for example, a biodiesel.

摘要翻译： 本文提供的是包含超过50％的中链脂肪酸或其脂肪酸烷基酯并具有低熔点的混合油或脂肪酸。 这种共混物可用作燃料或作为用于生产例如生物柴油的原料。 还提供遗传改变或修饰的植物，被修饰，使得植物产生的中链脂肪酸的量增加。 进一步提供了一种预测脂肪酸甲酯共混物的熔点的方法，以及用于鉴定适合用作例如生物柴油的共混物的方法。