公开(公告)号：US5871918A

公开(公告)日：1999-02-16

申请号：US667338

申请日：1996-06-20

申请人： H. Holden Thorp ,  Dean H. Johnston ,  Mary E. Napier ,  Carson R. Loomis ,  Mark F. Sistare ,  Jinheung Kim

发明人： H. Holden Thorp ,  Dean H. Johnston ,  Mary E. Napier ,  Carson R. Loomis ,  Mark F. Sistare ,  Jinheung Kim

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  C12Q1/6816 ,  C12Q1/6825

摘要： A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.

公开(公告)号：US06361951B1

公开(公告)日：2002-03-26

申请号：US09603217

申请日：2000-06-26

申请人： H. Holden Thorp ,  Dean H. Johnston ,  Mary E. Napier ,  Carson R. Loomis ,  Mark F. Sistare ,  Jinheung Kim

发明人： H. Holden Thorp ,  Dean H. Johnston ,  Mary E. Napier ,  Carson R. Loomis ,  Mark F. Sistare ,  Jinheung Kim

IPC分类号： C12Q168

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2563/113 ,  C12Q2525/197 ,  C12Q2525/117

摘要： A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.

公开(公告)号：US07049068B2

公开(公告)日：2006-05-23

申请号：US10008233

申请日：2001-11-06

申请人： H. Holden Thorp ,  Dean H. Johnston ,  Mary E. Napier ,  Carson R. Loomis ,  Mark F. Sistare ,  Jinheung Kim

发明人： H. Holden Thorp ,  Dean H. Johnston ,  Mary E. Napier ,  Carson R. Loomis ,  Mark F. Sistare ,  Jinheung Kim

IPC分类号： C12Q1/68 ,  C12M1/36 ,  G01N15/06 ,  C07H21/04

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2563/113 ,  C12Q2525/197 ,  C12Q2525/117

摘要： A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.

摘要翻译： 检测含有至少一个预选碱基（例如腺嘌呤，鸟嘌呤，6-巯基鸟嘌呤，8-氧代 - 鸟嘌呤和8-氧代腺嘌呤）的核酸（例如DNA，RNA）的方法包括：（a）使 具有能够在氧化还原反应中氧化预选碱的过渡金属络合物的核酸; （b）检测氧化还原反应; 和（c）从预选碱基处检测到的氧化还原反应确定核酸的存在或不存在。 该方法可用于多种应用，包括DNA测序，诊断测定和定量分析。

公开(公告)号：US6132971A

公开(公告)日：2000-10-17

申请号：US179665

申请日：1998-10-27

申请人： H. Holden Thorp ,  Dean H. Johnston ,  Mary E. Napier ,  Carson R. Loomis ,  Mark F. Sistare ,  Jinheung Kim

发明人： H. Holden Thorp ,  Dean H. Johnston ,  Mary E. Napier ,  Carson R. Loomis ,  Mark F. Sistare ,  Jinheung Kim

摘要： A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.

公开(公告)号：US5968745A

公开(公告)日：1999-10-19

申请号：US950503

申请日：1997-10-14

申请人： H. Holden Thorp ,  Carson R. Loomis ,  Mary E. Napier

发明人： H. Holden Thorp ,  Carson R. Loomis ,  Mary E. Napier

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： B82Y15/00 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6825

摘要： A polymer-electrode including (a) a substrate having a conductive working surface; and (b) a polymer layer on the conductive working surface. The polymer layer has a plurality of microfluidic reaction openings distributed throughout the layer. An oligonucleotide probe can be attached to the polymer layer and is available to capture target nucleic acid. A soluble mediator can diffuse freely and transfer electrons from the preselected base in the hybridized nucleic acid to the conductive working surface of the substrate. An electronic signal generated from the electron transfer reaction is detected and quantitated.

摘要翻译： 1.一种聚合物电极，包括（a）具有导电性工作面的基板; 和（b）导电工作表面上的聚合物层。 聚合物层具有分布在整个层中的多个微流体反应开口。 寡核苷酸探针可以连接到聚合物层并且可用于捕获靶核酸。 可溶性介体可以自由扩散，并将电子从杂交核酸中的预选碱转移到基底的导电工作表面。 从电子转移反应产生的电子信号被检测和定量。