公开(公告)号：US3622460A

公开(公告)日：1971-11-23

申请号：US3622460D

申请日：1968-05-31

Applicant: HAYASHIBARA CO

Inventor： MASUDA KAZUO ,  MITSUHASHI MASAKAZU ,  HIRAO MAMORU ,  SATO YOSHINORI ,  SUGIMOTO KANAME

IPC: C12N9/44 ,  C12D13/00

CPC classification number: C12N9/246 ,  Y10S435/822

Abstract: According to the present invention, a strain of Aerobacter aerogenes is engaged in a preliminary culture upon a streak culture and then one platinum loop of the resulting seed culture is inoculated into a liquid medium sterilized as usual, which medium contains ammonium salt as a nitrogen source and liquefied starch as a carbon source, and is cultured under the condition of pH 5-8 to produce a large amount of isoamylase.