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公开(公告)号：US3560345A
公开(公告)日：1971-02-02
申请号：US3560345D
申请日：1968-05-31
Applicant: HAYASHIBARA CO
Inventor： YOKOBAYASHI YASUYUKI , SUGIMOTO KANAME , SATO YOSHINORI
CPC classification number: C12N9/2451 , Y10S435/874
Abstract: A CULTURE MEDIUM, WHICH CONTAINS APPROPRIATE SOURCES OF CARBON AND NITROGEN AND MINERAL MATERIALS, IS INOCULATED WITH PSEUDOMONAS AMLYODERAMOSA (ATCC NO. 21,262) AND THE STRAIN IS CULTURED WITH SHAKING AT ABOUT 30*C. AFTER THE CULTURE, THE CULTURE FLUID OBTAINED IS CENTRIFUGED AND COLD ACETONE IS ADDED TO THE SUPERNATANT FLUID TO PRECIPITATE ISOMYLASE. THE ISOAMYLASE PRECIPITATED IS CENTRIFUGALLY COLLECTED AND DRIED IN VACUO TO YIELD THE ISOAMYLASE IN POWDERY FORM.
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