公开(公告)号：US20160244721A1

公开(公告)日：2016-08-25

申请号：US15028158

申请日：2014-10-09

Applicant: HEALIOS K.K. ,  OSAKA UNIVERSITY

Inventor： Masanori SAWADA ,  Kiyotoshi SEKIGUCHI

IPC: C12N5/079 ,  A61K35/30

CPC classification number: C12N5/0621 ,  A61K9/0048 ,  A61K35/30 ,  C12N2501/727 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2533/52

Abstract: The present invention provides a method of purifying highly pure retinal pigment epithelial cells from a cell population obtained by induction of differentiation of pluripotent stem cells into retinal pigment epithelial cells, by a simple and easy operation in a short period. The purification method of the present invention includes a step of introducing a cell population containing retinal pigment epithelial cells obtained by differentiation induction of pluripotent stem cells on laminin or a fragment thereof on a filter, and obtaining a cell population that passed the filter.

Abstract translation: 本发明提供了通过在短时间内简单且容易的操作诱导将多能干细胞分化为视网膜色素上皮细胞而获得的细胞群中纯化高纯度视网膜色素上皮细胞的方法。 本发明的纯化方法包括将通过将多能干细胞分化诱导获得的视网膜色素上皮细胞的细胞群导入到过滤器上的层粘连蛋白或其片段上，获得通过过滤器的细胞群的步骤。

公开(公告)号：US20160237403A1

公开(公告)日：2016-08-18

申请号：US15028076

申请日：2014-10-09

Applicant: HEALIOS K.K. ,  RIKEN ,  OSAKA UNIVERSITY

Inventor： Masanori SAWADA ,  Masayo TAKAHASHI ,  Kiyotoshi SEKIGUCHI

IPC: C12N5/079 ,  A61K9/00 ,  A61K35/30

CPC classification number: C12N5/0621 ,  A61K9/0048 ,  A61K35/30 ,  C12N2501/727 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2533/52

Abstract: Provided are a production method of retinal pigment epithelial (RPE) cells that improves differentiation induction efficiency of pluripotent stem cells into RPE cells, and can provide highly pure RPE cells by a simple and easy operation in a short period, a culture method of RPE cells that can stably grow and culture a cell, a toxicity/efficacy evaluation method using RPE cells useful for transplantation therapy, and a therapeutic drug for a retinal disease. The invention relates to a production method of RPE cells, comprising adhesion culture of human pluripotent stem cells using a culture substrate coated with a laminin-E8 fragment, a culture method of RPE cells, comprising adhesion culture of RPE cells using a culture substrate coated with a laminin-E8 fragment, a toxicity or efficacy evaluation method using RPE cells obtained by producing or culturing by the method, and a therapeutic drug for a retinal disease, containing the RPE cells.

Abstract translation: 提供了一种提高多能干细胞向RPE细胞分化诱导效率的视网膜色素上皮（RPE）细胞的制备方法，可以通过简单易用的操作在短时间内提供高纯度的RPE细胞，RPE细胞的培养方法 可以稳定地培养细胞，使用可用于移植治疗的RPE细胞的毒性/功效评价方法，以及用于视网膜疾病的治疗药物。 本发明涉及RPE细胞的制备方法，其包括使用包被有层粘连蛋白-E8片段的培养基底的人多能干细胞的粘附培养，RPE细胞的培养方法，包括使用涂覆有 层粘连蛋白E8片段，使用通过该方法生产或培养获得的RPE细胞的毒性或功效评价方法和含有RPE细胞的视网膜疾病治疗药。