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    METHOD OF PRODUCING RETINAL PIGMENT EPITHELIAL CELL
    1.
    发明申请
    METHOD OF PRODUCING RETINAL PIGMENT EPITHELIAL CELL 审中-公开
    生产细胞色素上皮细胞的方法

    公开(公告)号：US20160237403A1

    公开(公告)日：2016-08-18

    申请号：US15028076

    申请日：2014-10-09

    Applicant: HEALIOS K.K. ,  RIKEN ,  OSAKA UNIVERSITY

    Inventor： Masanori SAWADA ,  Masayo TAKAHASHI ,  Kiyotoshi SEKIGUCHI

    IPC: C12N5/079 ,  A61K9/00 ,  A61K35/30

    CPC classification number: C12N5/0621 ,  A61K9/0048 ,  A61K35/30 ,  C12N2501/727 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2533/52

    Abstract: Provided are a production method of retinal pigment epithelial (RPE) cells that improves differentiation induction efficiency of pluripotent stem cells into RPE cells, and can provide highly pure RPE cells by a simple and easy operation in a short period, a culture method of RPE cells that can stably grow and culture a cell, a toxicity/efficacy evaluation method using RPE cells useful for transplantation therapy, and a therapeutic drug for a retinal disease. The invention relates to a production method of RPE cells, comprising adhesion culture of human pluripotent stem cells using a culture substrate coated with a laminin-E8 fragment, a culture method of RPE cells, comprising adhesion culture of RPE cells using a culture substrate coated with a laminin-E8 fragment, a toxicity or efficacy evaluation method using RPE cells obtained by producing or culturing by the method, and a therapeutic drug for a retinal disease, containing the RPE cells.

    Abstract translation: 提供了一种提高多能干细胞向RPE细胞分化诱导效率的视网膜色素上皮（RPE）细胞的制备方法，可以通过简单易用的操作在短时间内提供高纯度的RPE细胞，RPE细胞的培养方法 可以稳定地培养细胞，使用可用于移植治疗的RPE细胞的毒性/功效评价方法，以及用于视网膜疾病的治疗药物。 本发明涉及RPE细胞的制备方法，其包括使用包被有层粘连蛋白-E8片段的培养基底的人多能干细胞的粘附培养，RPE细胞的培养方法，包括使用涂覆有 层粘连蛋白E8片段，使用通过该方法生产或培养获得的RPE细胞的毒性或功效评价方法和含有RPE细胞的视网膜疾病治疗药。

    THERAPEUTIC AGENT FOR DISEASE CAUSED BY DOMINANT MUTANT GENE
    2.
    发明申请
    THERAPEUTIC AGENT FOR DISEASE CAUSED BY DOMINANT MUTANT GENE 有权

    公开(公告)号：US20220249698A1

    公开(公告)日：2022-08-11

    申请号：US17626042

    申请日：2020-07-10

    Applicant: RIKEN

    Inventor： Masayo TAKAHASHI ,  Akishi ONISHI ,  Yuji TSUNEKAWA

    IPC: A61K48/00 ,  A61K38/46 ,  A61K31/7088 ,  A61K38/17 ,  A61P27/02

    Abstract: Provided is a novel therapeutic agent for a disease caused by a dominant gene mutation (7). A therapeutic agent of the present invention comprises a donor DNA (20) that contains a polynucleotide having the following sequences (a) to (c): (a) a normal gene (1); (b) a first reverse target sequence (2a) that is located upstream of the normal gene (1) and that is cleaved by a designer nuclease; and (c) a second reverse target sequence (2b) that is located downstream of the normal gene (1) and that is cleaved by the designer nuclease, where the reverse target sequences (2a, 2b) each mean a sequence obtained by inverting a target sequence (6) that is present in the genome and that is cleaved by the designer nuclease.

    CONTAINER FOR TRANSPORTING CELLS AND BIOTISSUE
    6.
    发明申请
    CONTAINER FOR TRANSPORTING CELLS AND BIOTISSUE 审中-公开

    公开(公告)号：US20190185799A1

    公开(公告)日：2019-06-20

    申请号：US16311588

    申请日：2017-06-01

    Applicant: SANPLATEC CORPORATION LTD. ,  RIKEN

    Inventor： Satoshi KATOU ,  Junichi KUWABARA ,  Masayo TAKAHASHI ,  Naoshi KOIDE

    IPC: C12M1/12 ,  C12M1/00 ,  C12M1/04 ,  B65D77/06

    CPC classification number: C12M23/06 ,  B65D77/06 ,  C12M1/00 ,  C12M3/00 ,  C12M23/22 ,  C12M23/24 ,  C12M23/38 ,  C12M23/52 ,  C12M37/00 ,  G01N1/28

    Abstract: The invention provides a cell and biotissue transporting container for transporting cells or biotissues while maintaining their cultured state. A cell/biotissue transporting container A10 includes a base member 1 with an opening 110 at an upper end and a first tubular portion 11 extending vertically, a lid body 2 including a top plate portion 21 to close the opening 110 and a second tubular portion 22 extending from a peripheral edge of the top plate portion 21 in the thickness direction to fit onto the first tubular portion 11, and a container body 3, formed separately from the base member 1 using a flexible material, where the container body includes a bottom wall portion 31, a tubular side wall portion 32 rising from a peripheral edge of the bottom wall portion 31 and inserted into the opening 110, and a flange 33 extending outward from an upper edge of the side wall portion 32. At least one of the first and second tubular portions 11 and 22 includes a lock preventing relative movement between the base member 1 and the lid body 2 with the flange 33 being clamped between a peripheral edge portion of the opening 110 of the base member and the top plate portion 21 of the lid body 2.

    METHOD FOR PRODUCING RETINAL TISSUES
    9.
    发明申请
    METHOD FOR PRODUCING RETINAL TISSUES 有权

    公开(公告)号：US20240399026A1

    公开(公告)日：2024-12-05

    申请号：US18805131

    申请日：2024-08-14

    Applicant: RIKEN ,  SUMITOMO PHARMA CO., LTD.

    Inventor： Daiki NUKAYA ,  Mototsugu EIRAKU ,  Yuiko KINOSE ,  Akishi ONISHI ,  Masayo TAKAHASHI ,  Yoshiki SASAI (deceased)

    IPC: A61L27/38 ,  C12N5/0793 ,  G01N33/50

    Abstract: The present invention aims to provide a method for suppressing differentiation of ganglion cell, amacrine cell, horizontal cell and/or bipolar cell in a neural retina tissue containing photoreceptor precursor and/or photoreceptor, and the like. A method for suppressing differentiation of a ganglion cell, an amacrine cell, a horizontal cell and/or a bipolar cell in a neural retinal tissue containing a photoreceptor precursor and/or a photoreceptor, including a step of culturing a retinal tissue comprising a neural retinal progenitor cell and in any stage between a differentiation stage immediately after emergence of a ganglion cell and a differentiation stage where emergence rate of a cone photoreceptor precursor reaches maximum in a medium containing a thyroid gland hormone signal transduction pathway agonist.

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