公开(公告)号：US11591617B2

公开(公告)日：2023-02-28

申请号：US16941780

申请日：2020-07-29

申请人： HELMHOLTZ ZENTRUM MUNCHEN - DEUTSCHES FORSCHUNGSZENTRUM FUR GESUNDHEIT UND UMWELT (GMBH) ,  SIRION BIOTECH GMBH

发明人： Natasa Anastasov ,  Ines Höfig ,  Christian Thirion

IPC分类号： C12N15/86 ,  C12N15/867 ,  A61K48/00

摘要： The present invention relates to a method for transducing a target cell, the method comprising the step of contacting a target cell with a retroviral vector and a poloxamer having a molecular weight of 12.8 kDa to about 15 kDa. Further, the invention relates to the use of a poloxamer as defined herein, optionally in combination with a polycationic substance as defined herein, for transducing a target cell with a retroviral vector and a kit comprising a retroviral vector, a poloxamer as defined herein and, optionally, instructions for use.

公开(公告)号：US10815498B2

公开(公告)日：2020-10-27

申请号：US15713422

申请日：2017-09-22

申请人： HELMHOLTZ ZENTRUM MUNCHEN-DEUTSCHES FORSCHUNGS-ZE ,  SIRION BIOTECH GMBH

发明人： Natasa Anastasov ,  Ines Höfig ,  Christian Thirion

IPC分类号： C12N15/867 ,  A61K48/00 ,  C12N15/86

摘要： The present invention relates to a method for transducing a target cell, the method comprising the step of contacting a target cell with a retroviral vector and a poloxamer having a molecular weight of 12.8 kDa to about 15 kDa. Further, the invention relates to the use of a poloxamer as defined herein, optionally in combination with a polycationic substance as defined herein, for transducing a target cell with a retroviral vector and a kit comprising a retroviral vector, a poloxamer as defined herein and, optionally, instructions for use.

公开(公告)号：US20150064788A1

公开(公告)日：2015-03-05

申请号：US14381586

申请日：2013-02-28

申请人： HELMHOLTZ ZENTRUM MÜNCHEN-DEUTSCHES FORSCHUNGS- ZENTRUM FÜR GESUNDHEIT UND UMWELT ,  Sirion Biotech GmbH

发明人： Natasa Anastasov ,  Ines Höfig ,  Christian Thirion

IPC分类号： C12N15/86

CPC分类号： C12N15/86 ,  A61K48/0041 ,  C12N15/867 ,  C12N2740/10041 ,  C12N2740/10043 ,  C12N2740/15043 ,  C12N2810/6081

摘要： The present invention relates to a method for transducing a target cell, the method comprising the step of contacting a target cell with a retroviral vector and a poloxamer having a molecular weight of 12.8 kDa to about 15 kDa. Further, the invention relates to the use of a poloxamer as defined herein, optionally in combination with a polycationic substance as defined herein, for transducing a target cell with a retroviral vector and a kit comprising a retroviral vector, a poloxamer as defined herein and, optionally, instructions for use.

摘要翻译： 本发明涉及一种用于转导靶细胞的方法，所述方法包括使靶细胞与逆转录病毒载体和分子量为12.8kDa至约15kDa的泊洛沙姆接触的步骤。 此外，本发明涉及如本文所定义的泊洛沙姆，任选地与本文定义的聚阳离子物质组合使用用于用逆转录病毒载体转导靶细胞的用途，以及包含逆转录病毒载体，本文定义的泊洛沙姆的试剂盒， 可选地，使用说明书。

公开(公告)号：US09771599B2

公开(公告)日：2017-09-26

申请号：US14381586

申请日：2013-02-28

申请人： HELMHOLTZ ZENTRUM MÜNCHEN-DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) ,  SIRION BIOTECH GMBH

发明人： Natasa Anastasov ,  Ines Höfig ,  Christian Thirion

IPC分类号： C12N15/86 ,  C12N15/867 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K48/0041 ,  C12N15/867 ,  C12N2740/10041 ,  C12N2740/10043 ,  C12N2740/15043 ,  C12N2810/6081

摘要： The present invention relates to a method for transducing a target cell, the method comprising the step of contacting a target cell with a retroviral vector and a poloxamer having a molecular weight of 12.8 kDa to about 15 kDa. Further, the invention relates to the use of a poloxamer as defined herein, optionally in combination with a polycationic substance as defined herein, for transducing a target cell with a retroviral vector and a kit comprising a retroviral vector, a poloxamer as defined herein and, optionally, instructions for use.