公开(公告)号：US20230073786A1

公开(公告)日：2023-03-09

申请号：US17467098

申请日：2021-09-03

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Yang LEI ,  Viktor SHKOLNIKOV ,  Daixi XIN

IPC: B03C5/02 ,  B03C5/00

Abstract: For some examples, an apparatus to effect particle separation may include a dielectrophoretic particle separator that may separate particles within a focused particle stream. The dielectrophoretic particle separator may apply an electric field to the particles. An imaging system may capture images of the particles passing through a separation region of the dielectrophoretic particle separator. An image processing and control system may process the images to obtain information regarding the particles and may adjust an operating parameter of the dielectrophoretic separator based upon the information regarding the particles.

公开(公告)号：US20230132556A1

公开(公告)日：2023-05-04

申请号：US17911559

申请日：2020-04-07

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor SHKOLNIKOV ,  Daixi XIN

IPC: C12M1/42 ,  C12N13/00

Abstract: A cell transfection apparatus may include a microfluidic chip. The microfluidic chip may include a fluid input port, a cell sorter to sort target cells from non-target cells in fluid received through the input port, a cell transfection region comprising a single cell electroporation region to receive the target cells sorted from the nontarget cells and a fluid ejector to dispense a transfected target cell received from the cell transfection region.

公开(公告)号：US20230017342A1

公开(公告)日：2023-01-19

申请号：US17954553

申请日：2022-09-28

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Daixi XIN

IPC: C12M3/06 ,  C12M1/00 ,  C12M1/42 ,  B01L3/00 ,  F04B19/00 ,  F04B43/04 ,  F04B43/06 ,  F04B9/103

Abstract: The present disclosure is drawn to microfluidic cellular membrane modification. In one example, a method of modifying cells can include pumping a fluid comprising cells in a forward direction through a microfluidic channel, applying an electric field within the microfluidic channel as cells flow in the forward direction through the electric field and beyond within the microfluidic channel, and pumping the fluid in a backward direction through the microfluidic channel, wherein cells flow in the backward direction returning through the electric field.