公开(公告)号：US10139390B2

公开(公告)日：2018-11-27

申请号：US14898298

申请日：2014-04-18

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Hirokazu Kato ,  Tomohiro Shoji

IPC: G01N33/487 ,  C12Q1/6869 ,  G01N27/447

Abstract: By slowing down the passing velocity of a DNA molecule in a nanopore, the accuracy of the reading of a nucleotide sequence of DNA is improved. A small temperature difference is introduced between a DNA molecule having an asymmetric and periodic structure and a nanopore membrane that carries the DNA molecule, whereby the DNA molecule that passes through a nanopore can move in one direction and the passing velocity of the DNA molecule in the nanopore can be controlled and reduced. In this manner, the accuracy of the analysis of a nucleotide sequence can be improved. Furthermore, it becomes possible to dissociate double-stranded DNA into single-stranded DNA molecules by the action of temperature and subject the single-stranded DNA molecules to a measurement selectively. Furthermore, it also becomes possible to select the polarity of a DNA molecule and subject the DNA molecule to a measurement.

公开(公告)号：US10168281B2

公开(公告)日：2019-01-01

申请号：US15539228

申请日：2015-11-11

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Tomohiro Shoji ,  Junji Shiokawa ,  Akihito Nishizawa ,  Hirokazu Kato ,  Junji Ishizuka

IPC: G01N21/64 ,  G01J3/44 ,  G01J3/36 ,  G01N21/03 ,  G01J3/10

Abstract: A multicolor fluorescence analysis device 11 is for detecting fluorescence emitted, as a result of excitation light irradiation, from a plurality of types of fluorophores included in a sample s, and is provided with an irradiation optical unit 520 for irradiating light emitted from a light source 510 onto a sample s as excitation light, a fluorescence condensation unit 530 having a fluorescence filter 531 that transmits light emitted from the sample s and transmits light of transmission wavelength bands different from the excitation wavelength bands, and a two-dimensional detector 554 that has a plurality of types of transmission filters 556 for transmitting prescribed wavelengths of light and detects the intensity of the light of the prescribed wavelength for each transmission filter 556, and the light emitted from at least two fluorophores from among the plurality of types of fluorophores is detected simultaneously and the fluorophore types are identified accordingly.