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1.发明申请MICROBIAL DETECTION APPARATUS, MICROBIAL DETECTION METHOD, AND SAMPLE CONTAINER USED THEREIN 有权微生物检测装置，微生物检测方法及其使用的样品容器公开(公告)号：US20140011221A1
公开(公告)日：2014-01-09
申请号：US14023640
申请日：2013-09-11
发明人： Masahiro OKANOJO , Hideyuki NODA , Noe MIYASHITA
摘要： The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes.
摘要翻译： 样品容器具有两层膜过滤器，其包括作为亲水膜过滤器的上层的第一层和作为第二层的疏水膜过滤器，其能够不使用润湿剂并且通过手段过滤水溶液 形成负压。 使用该样品容器，通过由抽吸部分形成的负压过滤大量的水样品溶液，以通过亲水膜过滤器捕获含水样品溶液中的微生物。 然后，将负压恢复到正常压力，然后将微生物溶解溶液加入到膜过滤器中，以将微生物溶解溶液在给定时间内保留在疏水膜过滤器上。 然后，将微生物溶解溶液分配到含有发光试剂的反应容器中，检测发光以检测微生物。
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