公开(公告)号：US20230181658A1

公开(公告)日：2023-06-15

申请号：US17994162

申请日：2023-02-13

申请人： HUAZHONG AGRICULTURAL UNIVERSITY

发明人： Yijie Chen ,  Jinyu Chen ,  Qi Zhou ,  Beixi Li ,  Qian Shen ,  Wei Zheng ,  Yilun Luo ,  Shuang Ding ,  Fei Han ,  Liuqing Yang

IPC分类号： A61K35/747 ,  A61K9/113 ,  A61K47/24 ,  A61K9/00 ,  A61K47/34

CPC分类号： A61K35/747 ,  A61K9/0095 ,  A61K9/113 ,  A61K47/24 ,  A61K47/34

摘要： A method for preparing a probiotic preparation based on a W1/O/W2 double emulsion structure includes steps of: using a solution containing probiotics or a mixed solution of probiotics and a probiotic protective agent as an internal water phase W1; dissolving an emulsifier in a lipid phase O, and mixing the lipid phase O with the internal water phase W1; then preparing a primary emulsion W1/O by stirring and low-energy emulsification or high-energy emulsification; and using an emulsifier-contained solution as an external water phase W2, and adding the external water phase W2 to the primary emulsion W1/O; then preparing a W1/O/W2 double emulsion by stirring and the low-energy emulsification or the high-energy emulsification, thereby obtaining the probiotic preparation. The double emulsion system is used to embed the probiotics, which can keep the probiotics active during low-temperature storage and freezing and thawing.