公开(公告)号：US10378052B2

公开(公告)日：2019-08-13

申请号：US14990825

申请日：2016-01-08

申请人： HUAZHONG AGRICULTURAL UNIVERSITY

发明人： Meizhong Luo ,  Yonglong Pan

IPC分类号： G01N33/48 ,  C12Q1/6874 ,  C12Q1/6806 ,  G16B30/00

摘要： A whole-genome sequencing method, including: 1) extracting a whole-genome DNA and constructing a BAC library; 2) constructing mixing pools; 3) extracting DNA of the mixing pools; 4) performing pair-end sequencing for the DNA from the mixing pools; 5) scanning sequences of each mixing pool to obtain a feature sequence set and a k-mer set of each mixing pool; 6) analyzing a corresponding feature sequence set and a corresponding k-mer set of each clone; 7) constructing clone contigs; 8) dividing the k-mer sets of the clones into small k-mer sets and positioning the small k-mer sets; 9) assembling the NGS sequences to yield sequence contigs; 10) positioning the sequence contigs, connecting the sequence contigs and determining directions thereof; and 11) determining relative positions and directions of the clone contigs, and connecting the sequences of clone contigs as whole stripe of chromosome sequences.

公开(公告)号：US20160194704A1

公开(公告)日：2016-07-07

申请号：US14990825

申请日：2016-01-08

申请人： HUAZHONG AGRICULTURAL UNIVERSITY

发明人： Meizhong Luo ,  Yonglong Pan

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  G16B30/00

摘要： A whole-genome sequencing method, including: 1) extracting a whole-genome DNA and constructing a BAC library; 2) constructing mixing pools; 3) extracting DNA of the mixing pools; 4) performing pair-end sequencing for the DNA from the mixing pools; 5) scanning sequences of each mixing pool to obtain a feature sequence set and a k-mer set of each mixing pool; 6) analyzing a corresponding feature sequence set and a corresponding k-mer set of each clone; 7) constructing clone contigs; 8) dividing the k-mer sets of the clones into small k-mer sets and positioning the small k-mer sets; 9) assembling the NGS sequences to yield sequence contigs; 10) positioning the sequence contigs, connecting the sequence contigs and determining directions thereof; and 11) determining relative positions and directions of the clone contigs, and connecting the sequences of clone contigs as whole stripe of chromosome sequences.

摘要翻译： 全基因组测序方法，包括：1）提取全基因组DNA，构建BAC文库; 2）建设混合池; 3）提取混合池的DNA; 4）对来自混合池的DNA进行成对测序; 5）扫描每个混合池的序列以获得每个混合池的特征序列集和k-聚集; 6）分析每个克隆的相应特征序列集和相应的k-mer集合; 7）构建克隆重叠群; 8）将克隆的k-mer集合分成小的k-mer集合并定位小的k-mer集合; 9）组装NGS序列以产生序列重叠群; 10）定位序列重叠群，连接序列重叠群及其确定方向; 和11）确定克隆重叠群的相对位置和方向，并将克隆重叠群的序列连接成染色体序列的整个条带。