公开(公告)号：US6046312A

公开(公告)日：2000-04-04

申请号：US436865

申请日：1999-11-09

申请人： Han-chul Song ,  Kwang-nak Koh ,  Shin-won Kang ,  Jin-ho Cho ,  Joohg-hun Kim ,  Su-mi Lee

发明人： Han-chul Song ,  Kwang-nak Koh ,  Shin-won Kang ,  Jin-ho Cho ,  Joohg-hun Kim ,  Su-mi Lee

IPC分类号： C12Q1/54 ,  C09B29/00 ,  C09B29/036 ,  C09B29/08 ,  C09B29/09 ,  C09B67/36 ,  G01N31/22 ,  G01N33/483 ,  G01N33/49 ,  G01N33/52 ,  G01N33/66

CPC分类号： C09B29/0048 ,  C09B29/0803 ,  G01N33/52 ,  G01N33/66 ,  Y10T436/144444

摘要： Disclosed are water soluble azo dyes, represented by Formula I or II, and their synthesis and use. The water soluble azo dyes are synthesized by diazotizing 4-aminopyridine and coupling the resulting diazo intermediate with alkylated metanilic or orthanilic acid. To be suitable for measuring blood glucose levels, the dyes are combined with m-nitrophenyl boronic acid in an aqueous solvent. A combined ao form of the dyes with glucose shows a peak at 540 nm. At this wavelength, the absorptivity has a tendency to increase with the increasing of glucose level, which allows the measurement of blood glucose levels. ##STR1## wherein R has a 1-4 carbon atom length and is an alkyl, a hydroxy alkyl, a carboxyl-containing alkyl, or a sulfonyl-containing alkyl.

摘要翻译： 公开了由式I或II表示的水溶性偶氮染料及其合成和用途。 通过重氮化4-氨基吡啶合成水溶性偶氮染料并将所得重氮中间体与烷基化的邻苯二甲酸或邻氨基苯甲酸偶合。 为了适合于测量血糖水平，将染料与间硝基苯基硼酸在水性溶剂中混合。 染料与葡萄糖的组合形式在540nm处显示峰。 在这种波长下，吸收率随葡萄糖水平的增加而增加，这允许测量血糖水平。 其中R具有1-4个碳原子的长度，并且是烷基，羟基烷基，含羧基的烷基或含磺酰基的烷基。