-
1.发明授权Method for preparing soluble and active recombinant proteins usins PDI as a fusion partner 有权制备可溶性和活性重组蛋白的方法使PDI作为融合配偶体公开(公告)号：US08080387B2
公开(公告)日：2011-12-20
申请号：US12091291
申请日：2005-10-24
申请人： Hang-Cheol Shin , Yean-Hee Park , Hyang-Do Song , Eung-Yoon Kim , Ha-A-Rin Chon , Hye-Ran Hyun
发明人： Hang-Cheol Shin , Yean-Hee Park , Hyang-Do Song , Eung-Yoon Kim , Ha-A-Rin Chon , Hye-Ran Hyun
CPC分类号： C12N15/62 , C07K2319/00 , C07K2319/21 , C12N9/90 , C12P21/02
摘要： The present invention relates to a method for producing a recombinant protein capable of increasing expression rate of a target protein and also improving solubility and folding of the expressed target protein using a modified protein disulfide isomerase (PDI) as a fusion partner, and an expression vector containing the modified PDI gene as a fusion partner. The method for preparing a recombinant protein using a modified PDI as a fusion partner according to the present invention may solve the problems concerning a low yield and solubility and folding that conventional fusion partners have, and be widely used for protein drug and industrial protein production.
摘要翻译： 本发明涉及能够提高靶蛋白表达率的重组蛋白质的制造方法，并且使用修饰的蛋白质二硫键异构酶（PDI）作为融合对象，提高表达的靶蛋白的溶解性和折叠性，以及表达载体 含有修饰的PDI基因作为融合配偶体。 使用修饰的PDI作为本发明的融合对象的重组蛋白质的制备方法可以解决常规的融合对象具有的低产率和溶解性以及折叠的问题，并被广泛用于蛋白质药物和工业蛋白质的生产。
-
2.发明申请METHOD FOR PREPARING SOLUBLE AND ACTIVE RECOMBINANT PROTEINS USING PDI AS A FUSION PARTNER 有权使用PDI作为融合合作伙伴制备可溶性和活性重组蛋白的方法公开(公告)号：US20090305351A1
公开(公告)日：2009-12-10
申请号：US12091291
申请日：2005-10-24
申请人： Hang-Cheol Shin , Yean-Hee Park , Hyang-Do Song , Eung-Yoon Kim , Ha-A-Rin Chon , Hye-Ran Hyun
发明人： Hang-Cheol Shin , Yean-Hee Park , Hyang-Do Song , Eung-Yoon Kim , Ha-A-Rin Chon , Hye-Ran Hyun
CPC分类号： C12N15/62 , C07K2319/00 , C07K2319/21 , C12N9/90 , C12P21/02
摘要： The present invention relates to a method for producing a recombinant protein capable of increasing expression rate of a target protein and also improving solubility and folding of the expressed target protein using a modified protein disulfide isomerase (PDI) as a fusion partner, and an expression vector containing the modified PDI gene as a fusion partner. The method for preparing a recombinant protein using a modified PDI as a fusion partner according to the present invention may solve the problems concerning a low yield and solubility and folding that conventional fusion partners have, and be widely used for protein drug and industrial protein production.
摘要翻译： 本发明涉及能够提高靶蛋白表达率的重组蛋白质的制造方法，并且使用修饰的蛋白质二硫键异构酶（PDI）作为融合对象，提高表达的靶蛋白的溶解性和折叠性，以及表达载体 含有修饰的PDI基因作为融合配偶体。 使用修饰的PDI作为本发明的融合对象的重组蛋白质的制备方法可以解决常规的融合对象具有的低产率和溶解性以及折叠的问题，并被广泛用于蛋白质药物和工业蛋白质的生产。
-
搜索结果
2 条记录 (耗时 0.038 秒)
