公开(公告)号：US5856463A

公开(公告)日：1999-01-05

申请号：US715568

申请日：1996-09-18

申请人： Hans Peter Blankenborg Prydz ,  Gaute Brede

发明人： Hans Peter Blankenborg Prydz ,  Gaute Brede

IPC分类号： C12N15/09 ,  A61K31/7105 ,  A61K38/00 ,  A61K48/00 ,  A61P9/10 ,  A61P37/06 ,  C12N5/10 ,  C12N9/00 ,  C12N9/12 ,  C12N15/113 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12N15/1137 ,  C12N9/1205 ,  C12Y207/01037 ,  A61K38/00 ,  C12N2310/121 ,  C12N2310/122 ,  C12N2310/321

摘要： Disclosed is a purified full-length cDNA molecule encoding putative serine kinase enzyme (PSKH-1), and the expression of the cDNA in a recombinant host cell to produce substantially purified PSKH-1, per se. Inactivation of PSKH-1 pre-MRNA or PSKH-1 mRNA halts DNA synthesis and cell division. Also disclosed are ribozymes capable of cleaving PSKH-1 pre-mRNA or mRNA and thus deactivating PSKH-1 translation. Ribozymes of the hammerhead and hairpin motifs, and various compositions containing same, are also disclosed. The ribozymes compositions are used in the treatment of mammalian patients suffering from diseases or medical conditions characterized by abnormal cell proliferation or growth such as cancer and various non-malignant diseases or medical conditions such as autoimmune diseases, allograft rejection and atherosclerosis.

摘要翻译： 公开了一种编码推定的丝氨酸激酶（PSKH-1）的纯化全长cDNA分子，以及cDNA在重组宿主细胞中的表达，以产生基本上纯化的PSKH-1本身。 PSKH-1前MRNA或PSKH-1 mRNA的失活停止DNA合成和细胞分裂。 还公开了能够切割PSKH-1前mRNA或mRNA并因此使PSKH-1翻译失活的核酶。 还公开了锤头和发夹基序的核酶和含有它们的各种组合物。 核酶组合物用于治疗患有以异常细胞增殖或生长为特征的疾病或医学病症的哺乳动物患者，例如癌症和各种非恶性疾病或医学病症如自身免疫疾病，同种异体移植排斥和动脉粥样硬化。