公开(公告)号：US08906605B2

公开(公告)日：2014-12-09

申请号：US13929548

申请日：2013-06-27

申请人： Hauh-Jyun Candy Chen ,  Yu-Chin Chen

发明人： Hauh-Jyun Candy Chen ,  Yu-Chin Chen

IPC分类号： C12Q1/00

CPC分类号： G01N33/6848

摘要： An analytical method of post-translational modifications in hemoglobin is disclosed. The analytical method comprises the steps of providing a blood comprising the hemoglobin with post-translational modification of nitration, nitrosylation, or oxidation; performing an extraction process to the blood by an organic solvent; quantifying the hemoglobin by a fluorescent spectrometry; hydrolyzing the hemoglobin into a plurality of peptides by an enzyme; and using a nanoflow liquid chromatography-nanospray ionization tandem mass spectrometry to characterize and quantify the post-translational modifications of the hemoglobin.

摘要翻译： 公开了血红蛋白翻译后修饰的分析方法。 分析方法包括以下步骤：提供包含血红蛋白的血液，其具有硝化，亚硝基化或氧化的翻译后修饰; 通过有机溶剂对血液进行提取处理; 通过荧光光谱法定量血红蛋白; 通过酶将血红蛋白水解成多种肽; 并使用纳流液相色谱 - 纳米喷雾电离串联质谱来表征和量化血红蛋白的翻译后修饰。