公开(公告)号：US11969717B2

公开(公告)日：2024-04-30

申请号：US17317470

申请日：2021-05-11

Applicant: Hebei University of Technology

Inventor： Yanjun Jiang ,  Liya Zhou ,  Saiguang Xue ,  Jing Gao

IPC: C12N9/16 ,  B01J21/08 ,  B01J21/18 ,  B01J23/75 ,  B01J23/755 ,  B01J31/00 ,  B01J35/00 ,  B01J37/00 ,  B01J37/04 ,  C07C209/32 ,  C07C201/06 ,  C07C205/22

CPC classification number: B01J31/003 ,  B01J21/08 ,  B01J21/18 ,  B01J23/75 ,  B01J23/755 ,  B01J35/008 ,  B01J35/0086 ,  B01J37/009 ,  B01J37/04 ,  C07C209/325 ,  C12N9/16 ,  C07C201/06 ,  C07C205/22 ,  C07K2319/21

Abstract: The present disclosure provides a method for preparing an organophosphorus degrading enzyme based multifunctional catalyst and an organophosphorus degrading enzyme based multifunctional catalyst and use thereof. In the present disclosure, the preparation method includes: directly adding a composite yolk-shell-structured nanomaterial into a crude enzyme solution of organophosphorus degrading enzyme with an affinity tag, and mixing, to obtain a mixture, and then subjecting the mixture to a separation, to obtain an organophosphorus degrading enzyme based multifunctional catalyst. According to the present disclosure, the method for preparing an organophosphorus degrading enzyme based multifunctional catalyst is simple in operation, and has a low cost; the multifunctional catalyst prepared by the same has low requirement for the purity of enzyme, support of which could be directionally binded with enzyme, and could be used for detecting an organophosphorus pesticide, and also for a cascade degradation of an organophosphorus pesticide. The final product p-aminophenol has important application value.

公开(公告)号：US20210346876A1

公开(公告)日：2021-11-11

申请号：US17317470

申请日：2021-05-11

Applicant: Hebei University of Technology

Inventor： Yanjun Jiang ,  Liya Zhou ,  Saiguang Xue ,  Jing Gao

IPC: B01J31/00 ,  B01J35/00 ,  B01J37/04 ,  B01J37/00 ,  B01J23/75 ,  B01J23/755 ,  B01J21/08 ,  B01J21/18

Abstract: The present disclosure provides a method for preparing an organophosphorus degrading enzyme based multifunctional catalyst and an organophosphorus degrading enzyme based multifunctional catalyst and use thereof. In the present disclosure, the preparation method includes: directly adding a composite yolk-shell-structured nanomaterial into a crude enzyme solution of organophosphorus degrading enzyme with an affinity tag, and mixing, to obtain a mixture, and then subjecting the mixture to a separation, to obtain an organophosphorus degrading enzyme based multifunctional catalyst. According to the present disclosure, the method for preparing an organophosphorus degrading enzyme based multifunctional catalyst is simple in operation, and has a low cost; the multifunctional catalyst prepared by the same has low requirement for the purity of enzyme, support of which could be directionally binded with enzyme, and could be used for detecting an organophosphorus pesticide, and also for a cascade degradation of an organophosphorus pesticide. The final product p-aminophenol has important application value.