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    Methods and compositions for targeting sequences of interest to the chloroplast
    1.
    发明授权
    Methods and compositions for targeting sequences of interest to the chloroplast 有权
    靶向叶绿体感兴趣序列的方法和组合物

    公开(公告)号：US09139842B2

    公开(公告)日：2015-09-22

    申请号：US13208960

    申请日：2011-08-12

    申请人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    发明人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    IPC分类号： C12N15/82 ,  C12N9/02

    CPC分类号： C12N15/8221 ,  C12N9/0069 ,  C12N15/82 ,  C12N15/8274 ,  C12Y113/11027

    摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.

    摘要翻译： 提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上 活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。 还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。 组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。 这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。 进一步提供包含这些序列的细胞，植物细胞，植物和种子。 还提供了使用各种序列的方法。

    Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast
    2.
    发明申请
    Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast 有权
    用于靶向叶绿体感兴趣的方法和组合物

    公开(公告)号：US20120042412A1

    公开(公告)日：2012-02-16

    申请号：US13208960

    申请日：2011-08-12

    申请人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    发明人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    IPC分类号： A01H1/06 ,  C12N15/82 ,  C12N5/10 ,  C12N9/96 ,  A01H5/10 ,  C07K19/00 ,  C12N9/02 ,  C12N15/62 ,  A01H5/00

    CPC分类号： C12N15/8221 ,  C12N9/0069 ,  C12N15/82 ,  C12N15/8274 ,  C12Y113/11027

    摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.

    摘要翻译： 提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上 活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。 还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。 组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。 这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。 进一步提供包含这些序列的细胞，植物细胞，植物和种子。 还提供了使用各种序列的方法。

    CHIMERIC PROMOTERS AND METHODS OF USE
    5.
    发明申请
    CHIMERIC PROMOTERS AND METHODS OF USE 有权
    CHIMERIC PROMOTERS和使用方法

    公开(公告)号：US20120042414A1

    公开(公告)日：2012-02-16

    申请号：US13209017

    申请日：2011-08-12

    申请人： Henrik Albert ,  Linda A. Castle ,  Jian Lu ,  Yumin Tao

    发明人： Henrik Albert ,  Linda A. Castle ,  Jian Lu ,  Yumin Tao

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N15/82 ,  C12N15/63

    CPC分类号： C12N15/8221 ,  C12N9/0069 ,  C12N15/82 ,  C12N15/8274 ,  C12Y113/11027

    摘要： Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.

    摘要翻译： 提供了包含来自4-羟基苯基丙酮酸双加氧酶（HPPD）基因及其活性变体和片段的启动子的组合物和方法，以及采用HPPD启动子调节区的嵌合启动子。 还提供了包含本文公开的各种启动子的表达盒和植物，其与感兴趣的多核苷酸可操作地连接。 进一步提供了使用本文所述的各种启动子来调节感兴趣的多核苷酸的表达的方法。

    Chimeric promoters and methods of use
    6.
    发明授权
    Chimeric promoters and methods of use 有权
    嵌合启动子和使用方法

    公开(公告)号：US08993837B2

    公开(公告)日：2015-03-31

    申请号：US13209017

    申请日：2011-08-12

    申请人： Henrik Albert ,  Linda A. Castle ,  Jian Lu ,  Yumin Tao

    发明人： Henrik Albert ,  Linda A. Castle ,  Jian Lu ,  Yumin Tao

    IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N15/00 ,  C12N5/10 ,  C07H21/04 ,  C12N9/02

    CPC分类号： C12N15/8221 ,  C12N9/0069 ,  C12N15/82 ,  C12N15/8274 ,  C12Y113/11027

    摘要： Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.

    摘要翻译： 提供了包含来自4-羟基苯基丙酮酸双加氧酶（HPPD）基因及其活性变体和片段的启动子的组合物和方法，以及采用HPPD启动子调节区的嵌合启动子。 还提供了包含本文公开的各种启动子的表达盒和植物，其与感兴趣的多核苷酸可操作地连接。 进一步提供了使用本文所述的各种启动子来调节感兴趣的多核苷酸的表达的方法。