公开(公告)号：US4686368A

公开(公告)日：1987-08-11

申请号：US728965

申请日：1985-04-30

申请人： Herbert L. Anderson ,  W. Wayne Kinnison ,  John W. Lillberg

发明人： Herbert L. Anderson ,  W. Wayne Kinnison ,  John W. Lillberg

IPC分类号： G01N27/447 ,  G01T1/29 ,  H01J47/06 ,  G01T1/00

CPC分类号： G01T1/2942 ,  G01N27/44717 ,  G01T1/2935 ,  H01J47/062

摘要： Apparatus and method for electronically reading planar two dimensional .beta.-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge of the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the .beta.-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700 .mu.m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally be obtained in less than one hour.

摘要翻译： 用于电子阅读平面二维β-射线发射体标记凝胶电泳图的装置和方法。 单个扁平的矩形多线比例室被放置成紧邻凝胶并且组件放置在与比例室的矩形面垂直的方式设置的强烈均匀的磁场中。 在比例室的方向上发射的β射线被执行螺旋运动，其基本上保留了它们在凝胶中原点坐标的知识。 垂直取向的平行线，平行平面阴极以电子方式感测由在其间产生的并联线阳极的作用而产生的电子雪崩效应的检测气体中由此产生的电离所产生的β-射线的位置。 当从入射在比例室的宇宙射线产生信号时，闪烁体允许本装置变得不敏感。 凝胶中放射性化合物浓度的分辨率超过700微米。 本发明的装置和方法代表了在数据采集的动态范围，线性和灵敏度方面的常规放射自显影技术的显着改进。 通常可以在不到一小时内获得具有显着浓度的标记化合物和/或高放射性标记核素的凝胶电泳图的浓度和位置图。