公开(公告)号：US20040021065A1

公开(公告)日：2004-02-05

申请号：US10333003

申请日：2003-01-14

发明人： Simone Weber

IPC分类号： G12B013/00

CPC分类号： G21G4/08 ,  A61B6/037 ,  A61B6/583 ,  G01T1/169 ,  G01T1/2942 ,  G21G4/06

摘要： The invention relates to a phantom for testing nuclear medicine instruments, such as for positron emission tomographs (PET), single-photon emission computed tomographs (SPECT) or even for autoradiography. The phantom contains a solid body that spontaneously emits gamma radiation or positrons. The disadvantages of a phantom filled with a radioactive liquid can be routinely overcome therewith. The inventive phantom can be produced with structuring smaller than 1 mm, and in particular even smaller than 0.1 mm. The inventive phantom can be used for the first time both for positron emission tomography (PET) and for autoradiography, since the phantom can emit both gamma radiation and positron radiation.

摘要翻译： 本发明涉及用于测试核医学仪器的体模，例如用于正电子发射断层摄影术（PET），单光子发射计算机断层摄影术（SPECT）或甚至用于放射自显影。 幻影包含一个自发发射γ辐射或正电子的固体。 填充有放射性液体的幻影的缺点可以常规地克服。 本发明的体模可以以小于1mm，特别是甚至小于0.1mm的结构生产。 本发明的体模可以首次用于正电子发射断层摄影（PET）和放射自显影，因为体模可以发射伽马辐射和正电子辐射。

公开(公告)号：US5830629A

公开(公告)日：1998-11-03

申请号：US652401

申请日：1996-05-23

申请人： Douglas L. Vizard ,  David J. Steklenski

发明人： Douglas L. Vizard ,  David J. Steklenski

IPC分类号： G03C1/725 ,  C09K11/00 ,  G01T1/29 ,  G03C1/73 ,  G03C1/76

CPC分类号： G01T1/2942

摘要： An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological sample in contact with a phosphor layer, a film layer and a reflector layer. The phosphor layer is preferably a gadolinium oxysulfide containing layer and the film is preferably a photographic film containing a tabular grain emulsion.

摘要翻译： 一种用于放射自显影的组合物，其顺序为包含与磷光体层接触的放射性标记的生物样品的样品层，膜层和反射层。 荧光体层优选为含硫氧化硫的层，并且膜优选为含有片状颗粒乳剂的照相胶片。

公开(公告)号：US4941092A

公开(公告)日：1990-07-10

申请号：US866355

申请日：1986-05-23

申请人： Makoto Hara ,  Masakazu Hashiue ,  Kazuyoshi Tanaka

发明人： Makoto Hara ,  Masakazu Hashiue ,  Kazuyoshi Tanaka

IPC分类号： C12Q1/68 ,  G01T1/29

CPC分类号： G01T1/2942 ,  C12Q1/6869

摘要： A signal processing method for determining base sequence of nucleic acids by subjecting digital signals to signal processing, said digital signals corresponding to an autoradiograph of plural resolved rows which are formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium,which comprises steps of:(1) detecting at least two bands continuously in the lower part of each resolved row and numbering the bands consecutively in order from the lower end;(2) obtaining correlation of a distance between the detected bands in the resolving direction with the band's number and predicting positions of undetected bands in the resolving direction from the correlation;(3) detecting at least one band on the resolved rows on the basis of the predicted positions and numbering the bands consecutively;(4) obtaining the correlation of the distance between the bands with the band's number for the already detected bands including the band newly detected in the step (3), and predicting positions of undetected bands from the correlation; and(5) repeating in order the steps (3) and (4) to thereby detect all bands on the resolved rows.

摘要翻译： 一种用于通过使数字信号进行信号处理来确定核酸碱基序列的信号处理方法，所述数字信号对应于通过分解碱基特异性DNA片段或碱基特异性RNA片段的混合物而形成的多个分辨行的放射自显影图 在支撑介质上具有一维方向的放射性元素，其包括以下步骤：（1）在每个分辨行的下部连续检测至少两个频带，并从下端依次对频带进行编号; （2）获得分辨方向上检测的频带之间的距离与频带数的相关性，并从相关性预测解析方向上的未检测频带的位置; （3）基于预测位置检测解决的行上的至少一个频带，并连续编号频带; （4）获得包括在步骤（3）中新检测的带的已经检测的频带的频带之间的距离与带的距离的相关性，以及从相关性预测未检测到的频带的位置; 和（5）按顺序重复步骤（3）和（4），从而检测解决的行上的所有频带。

公开(公告)号：US20170010369A1

公开(公告)日：2017-01-12

申请号：US15116173

申请日：2015-02-03

申请人： THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA ,  INVICRO, LLC

发明人： Harrison H. BARRETT ,  Brian MILLER ,  Yijun DING ,  Liying CHEN ,  John William HOPPIN ,  Luca CAUCCI

IPC分类号： G01T1/29 ,  A61B6/03 ,  A61B6/00 ,  G01T1/161

CPC分类号： G01T1/2942 ,  A61B6/037 ,  A61B6/4258 ,  A61B6/5205 ,  G01T1/161 ,  G01T1/2985 ,  G01T5/08

摘要： The present invention provides methods and systems for 3D imaging of in vivo and ex vivo tissues. The disclosed systems and methods employ an autoradiographic approach where particles emitted by a radioactive composition within the tissue are detected. Once detected, a 3D representation of the source of particles within the tissue is reconstructed for viewing and analysis.

摘要翻译： 本发明提供用于体内和离体组织的3D成像的方法和系统。 所公开的系统和方法采用放射自显影方法，其中检测由组织内的放射性组合物发射的颗粒。 一旦检测到，组织内的颗粒源的3D表示被重建用于观察和分析。

公开(公告)号：US06229147B1

公开(公告)日：2001-05-08

申请号：US09051260

申请日：1998-09-03

申请人： George William Fraser ,  John Ernest Lees

发明人： George William Fraser ,  John Ernest Lees

IPC分类号： H01L31115

CPC分类号： G01T1/28 ,  G01T1/2942

摘要： There is disclosed an autoradiography system which includes an autoradiography sample, a substantially radioisotope free microchannel plate (MCP), and an MCP signal collection means, wherein the MCP directly measures beta particles from radioisotopes within the sample.

摘要翻译： 公开了一种放射自显影系统，其包括放射自显影样品，基本上不含放射性同位素的微通道板（MCP）和MCP信号采集装置，其中MCP直接测量样品内放射性同位素的β粒子。

公开(公告)号：US5520182A

公开(公告)日：1996-05-28

申请号：US201491

申请日：1994-02-24

申请人： Stephen B. Leighton ,  James L. Olds

发明人： Stephen B. Leighton ,  James L. Olds

IPC分类号： A61B6/00 ,  G01N27/447 ,  G01T1/29

CPC分类号： G01T1/161 ,  A61B6/4057 ,  A61B6/4258 ,  A61B6/482 ,  G01N27/44717 ,  G01T1/2942 ,  A61B6/4007 ,  A61B6/4035

摘要： A two- and three-dimensional autoradiographical imaging system is provided which includes a charge coupled device for detecting the emission of radioactively labeled substances from materials such as tissue samples, brains of humans or animals, or substances used in electrophoresis applications. In a first aspect, a radioactively labeled substance is included in a tissue sample. The tissue sample is sequentially imaged by a charge coupled device and a sectioning tool such as a microtome to produce a plurality of two-dimensional images. A three-dimensional image of the tissue sample is generated by further processing of the plurality of two-dimensional images derived from the charge coupled device. In a further aspect of the invention, a charge coupled device is utilized to provide realtime imaging of metabolic or physiological parameters involved in brain activity. A charge coupled device is positioned adjacent a portion of brain tissue desired to be examined, the brain tissue having a radioactively labeled substance therein for detection by the charge coupled device. A two-dimensional realtime image is produced using the charge coupled device for use in clinical or behavioral studies. In another aspect of the invention, a charge coupled device is utilized in electrophoresis applications to monitor radioactively labeled or tagged substances during electrophoresis. In this application, use of the charge coupled device permits small sample volumes of material to be used during electrophoresis, as well as automatic end point detection.

摘要翻译： 提供一种二维和三维放射自显影成像系统，其包括用于检测来自诸如组织样品，人类或动物的大脑或用于电泳应用的物质的材料的放射性标记物质的发射的电荷耦合装置。 在第一方面，放射性标记的物质包括在组织样品中。 组织样品通过电荷耦合器件和切片工具（如切片机）顺序成像，以产生多个二维图像。 通过进一步处理从电荷耦合器件导出的多个二维图像来生成组织样本的三维图像。 在本发明的另一方面，电荷耦合器件用于提供参与脑活动的代谢或生理参数的实时成像。 电荷耦合器件邻近想要检查的脑组织的一部分定位，其中具有放射性标记的物质的脑组织被电荷耦合器件检测。 使用电荷耦合装置产生用于临床或行为研究的二维实时图像。 在本发明的另一方面，电荷耦合器件用于电泳应用中以在电泳期间监测放射性标记或标记的物质。 在该应用中，电荷耦合器件的使用允许在电泳过程中使用小的样品体积的材料，以及自动终点检测。

公开(公告)号：US5301671A

公开(公告)日：1994-04-12

申请号：US761157

申请日：1991-09-17

申请人： Stephen B. Leighton ,  James L. Olds

发明人： Stephen B. Leighton ,  James L. Olds

IPC分类号： A61B6/00 ,  G01N27/447 ,  G01T1/29 ,  A61B5/00

CPC分类号： G01T1/161 ,  A61B6/4057 ,  A61B6/4258 ,  A61B6/482 ,  G01N27/44717 ,  G01T1/2942 ,  A61B6/4007 ,  A61B6/4035

摘要： A two- and three-dimensional autoradiographical imaging system is provided which includes a charge coupled device for detecting the emission of radioactively labeled substances from materials such as tissue samples, brains of humans or animals, or substances used in electrophoresis applications. In a first aspect, a radioactively labeled substance is included in a tissue sample. The tissue sample is sequentially imaged by a charge coupled device and a sectioning tool such as a microtome to produce a plurality of two-dimensional images. A three-dimensional image of the tissue sample is generated by further processing of the plurality of two-dimensional images derived from the charge coupled device. In a further aspect of the invention, a charge coupled device is utilized to provide realtime imaging of metabolic or physiological parameters involved in brain activity. A charge coupled device is positioned adjacent a portion of brain tissue desired to be examined, the brain tissue having a radioactively labeled substance therein for detection by the charge coupled device. A two-dimensional realtime image is produced using the charge coupled device for use in clinical or behavioral studies.

摘要翻译： 提供一种二维和三维放射自显影成像系统，其包括用于检测来自诸如组织样品，人类或动物的大脑或用于电泳应用的物质的材料的放射性标记物质的发射的电荷耦合装置。 在第一方面，放射性标记的物质包括在组织样品中。 组织样品通过电荷耦合器件和切片工具（如切片机）顺序成像，以产生多个二维图像。 通过进一步处理从电荷耦合器件导出的多个二维图像来生成组织样本的三维图像。 在本发明的另一方面，电荷耦合器件用于提供参与脑活动的代谢或生理参数的实时成像。 电荷耦合器件邻近想要检查的脑组织的一部分定位，其中具有放射性标记的物质的脑组织被电荷耦合器件检测。 使用电荷耦合装置产生用于临床或行为研究的二维实时图像。

公开(公告)号：US5011781A

公开(公告)日：1991-04-30

申请号：US387506

申请日：1989-07-31

申请人： Coleman Bess

发明人： Coleman Bess

IPC分类号： C09K11/06 ,  G01T1/29

CPC分类号： G01T1/2942 ,  C09K11/06 ,  Y10S436/804

摘要： Disclosed is an improved autoradiography enhancer composition and method of use. The enhancer composition uses an acid anhydride as a dehydration agent.

摘要翻译： 公开了改进的放射自显影增强剂组合物和使用方法。 增强剂组合物使用酸酐作为脱水剂。

公开(公告)号：US4865967A

公开(公告)日：1989-09-12

申请号：US127605

申请日：1987-11-30

申请人： Hisashi Shiraishi ,  Junji Miyahara ,  Hisatoyo Kato

发明人： Hisashi Shiraishi ,  Junji Miyahara ,  Hisatoyo Kato

IPC分类号： G01N33/50 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/58 ,  G01N33/60 ,  G01T1/29

CPC分类号： G01T1/2942 ,  C12Q1/6841

摘要： An autoradiographic gene-screening method employing a hybridization process, which comprises:(1) a step of transferring a portion of genetic clones cultured on a culture medium onto a filter to fix them thereonto;(2) a step of hybridizing the genes of said clones fixed onto said filter with radioactively labeled probes;(3) a step of obtaining two dimensional information on the location of the radioactively labeled substances on the filter which comprises placing said filter having been subjected to the hybridization and a stimulable phosphor sheet in layers for a given period of time to cause said stimulable phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the filter, exciting said sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission to obtain a locational information on the radioactively labeled substances on the filter; and(4) a step recovering the clones on the culture medium according to the obtained locational information.

摘要翻译： 使用杂交方法的放射自显影基因筛选方法，其包括：（1）将在培养基上培养的一部分遗传克隆转移到过滤器上以将其固定在其上的步骤; （2）将固定在所述过滤器上的所述克隆的基因与放射性标记的探针杂交的步骤; （3）获得关于过滤器上的放射性标记物质的位置的二维信息的步骤，该步骤包括将已经进行了杂交的所述过滤器和可刺激的磷光体片层压一段给定的时间以引起所述可刺激的荧光体 以吸收由过滤器上的放射性标记的物质发射的辐射能量的至少一部分，用电磁波激发所述片材，以释放作为受激发射存储在所述片材中的辐射能量，并且检测受激发射以获得位置信息 在过滤器上的放射性标记物质上; 和（4）根据获得的位置信息恢复培养基上的克隆的步骤。

公开(公告)号：US4686368A

公开(公告)日：1987-08-11

申请号：US728965

申请日：1985-04-30

申请人： Herbert L. Anderson ,  W. Wayne Kinnison ,  John W. Lillberg

发明人： Herbert L. Anderson ,  W. Wayne Kinnison ,  John W. Lillberg

IPC分类号： G01N27/447 ,  G01T1/29 ,  H01J47/06 ,  G01T1/00

CPC分类号： G01T1/2942 ,  G01N27/44717 ,  G01T1/2935 ,  H01J47/062

摘要： Apparatus and method for electronically reading planar two dimensional .beta.-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge of the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the .beta.-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700 .mu.m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally be obtained in less than one hour.

摘要翻译： 用于电子阅读平面二维β-射线发射体标记凝胶电泳图的装置和方法。 单个扁平的矩形多线比例室被放置成紧邻凝胶并且组件放置在与比例室的矩形面垂直的方式设置的强烈均匀的磁场中。 在比例室的方向上发射的β射线被执行螺旋运动，其基本上保留了它们在凝胶中原点坐标的知识。 垂直取向的平行线，平行平面阴极以电子方式感测由在其间产生的并联线阳极的作用而产生的电子雪崩效应的检测气体中由此产生的电离所产生的β-射线的位置。 当从入射在比例室的宇宙射线产生信号时，闪烁体允许本装置变得不敏感。 凝胶中放射性化合物浓度的分辨率超过700微米。 本发明的装置和方法代表了在数据采集的动态范围，线性和灵敏度方面的常规放射自显影技术的显着改进。 通常可以在不到一小时内获得具有显着浓度的标记化合物和/或高放射性标记核素的凝胶电泳图的浓度和位置图。