Fluorescence lifetime distribution image measuring system and its measuring method
    2.
    发明申请
    Fluorescence lifetime distribution image measuring system and its measuring method 有权
    荧光寿命分布图像测量系统及其测量方法

    公开(公告)号:US20050157292A1

    公开(公告)日:2005-07-21

    申请号:US10516076

    申请日:2003-05-28

    IPC分类号: G01N21/64

    CPC分类号: G01N21/6408

    摘要: Pulse excitation light, emitted from a laser light source 10, is scanned in a first direction by a first scanning means 100, scanned in a second direction, perpendicular to the first direction, by a second scanning means 120, converged by an objective optical system 140, and illuminated onto sample 50. Fluorescences, emitted from sample 50, are output from objective optical system 140 to second scanning means 120, scanned in the second direction, perpendicular to the first direction, and output to a light separation means 110 by second scanning means 120, output from light separation means 110 to a streak camera, and recorded as variations of time of the fluorescence intensities by streak camera 30. Fluorescence lifetimes are calculated based on these variations with time of the fluorescence intensities and a fluorescence lifetime distribution image is prepared.

    摘要翻译: 由激光光源10发射的脉冲激发光由第一扫描装置100沿第一方向扫描,第一扫描装置100通过第二扫描装置120沿第二方向垂直于第一方向扫描,由物镜光学系统 140,并照射到样品50上。 从样品50发射的荧光从物镜光学系统140输出到与第一方向垂直扫描的第二扫描装置120,并通过第二扫描装置120输出到光分离装置110,从光分离 意味着110到条纹相机,并且被条纹相机30的荧光强度的时间变化记录下来。 基于荧光强度随时间的变化计算荧光寿命并制备荧光寿命分布图像。

    Fluorescence lifetime distribution image measuring system and its measuring method
    3.
    发明授权
    Fluorescence lifetime distribution image measuring system and its measuring method 有权
    荧光寿命分布图像测量系统及其测量方法

    公开(公告)号:US07453567B2

    公开(公告)日:2008-11-18

    申请号:US10516076

    申请日:2003-05-28

    IPC分类号: G01J3/30 G01J3/28

    CPC分类号: G01N21/6408

    摘要: Pulse excitation light, emitted from a laser light source 10, is scanned in a first direction by a first scanning means 100, scanned in a second direction, perpendicular to the first direction, by a second scanning means 120, converged by an objective optical system 140, and illuminated onto sample 50. Fluorescences, emitted from sample 50, are output from objective optical system 140 to second scanning means 120, scanned in the second direction, perpendicular to the first direction, and output to a light separation means 110 by second scanning means 120, output from light separation means 110 to a streak camera, and recorded as variations of time of the fluorescence intensities by streak camera 30. Fluorescence lifetimes are calculated based on these variations with time of the fluorescence intensities and a fluorescence lifetime distribution image is prepared.

    摘要翻译: 由激光光源10发射的脉冲激发光由第一扫描装置100沿第一方向扫描,第一扫描装置100通过第二扫描装置120沿第二方向垂直于第一方向扫描,由物镜光学系统 140,并照射到样品50上。 从样品50发射的荧光从物镜光学系统140输出到与第一方向垂直扫描的第二扫描装置120,并通过第二扫描装置120输出到光分离装置110,从光分离 意味着110到条纹相机,并且被条纹相机30的荧光强度的时间变化记录下来。 基于荧光强度随时间的变化计算荧光寿命并制备荧光寿命分布图像。