公开(公告)号：US20060289736A1

公开(公告)日：2006-12-28

申请号：US11442113

申请日：2006-05-30

申请人： Hiromichi Yamashita ,  Akihiro Takeda ,  Tomoyuki Kurosawa ,  Kinya Kobayashi

发明人： Hiromichi Yamashita ,  Akihiro Takeda ,  Tomoyuki Kurosawa ,  Kinya Kobayashi

IPC分类号： B01D59/44

CPC分类号： H01J49/0031 ,  G01N33/6848

摘要： The present invention achieves a mass analysis method that can identify protein or peptide with high speed and high sensitivity. A mass spectrum is obtained from a standard sample of healthy person, an ion is selected from the mass spectrum as a precursor ion, and a mass spectrum of the precursor ion is obtained [(a) to (d)]. A mass spectrum is obtained from a sample of interest to be measured of patient, an ion other than the precursor ion of the standard sample is selected from the mass spectrum as a precursor ion, and a mass spectrum of the precursor ion is obtained [(g) to (k)]. Identifications on peptide/protein specific to the standard sample and the sample to be measured, and common to both are conducted [(r) to (q)], and based on the results, comparative analysis on peptide/protein of the sample to be measured (t). Without regarding an ion derived from all components of the sample to be measured as precursor ion, MS/MS spectrum is obtained and identification on a plurality of components of the sample to be measured can be conducted for a short time and with high sensitivity.

公开(公告)号：US07485852B2

公开(公告)日：2009-02-03

申请号：US11442113

申请日：2006-05-30

申请人： Hiromichi Yamashita ,  Akihiro Takeda ,  Tomoyuki Kurosawa ,  Kinya Kobayashi

发明人： Hiromichi Yamashita ,  Akihiro Takeda ,  Tomoyuki Kurosawa ,  Kinya Kobayashi

IPC分类号： B01D59/44

CPC分类号： H01J49/0031 ,  G01N33/6848

摘要： The present invention achieves a mass analysis method that can identify protein or peptide with high speed and high sensitivity. A mass spectrum is obtained from a standard sample of healthy person, an ion is selected from the mass spectrum as a precursor ion, and a mass spectrum of the precursor ion is obtained [(a) to (d)]. A mass spectrum is obtained from a sample of interest to be measured of patient, an ion other than the precursor ion of the standard sample is selected from the mass spectrum as a precursor ion, and a mass spectrum of the precursor ion is obtained [(g) to (k)]. Identifications on peptide/protein specific to the standard sample and the sample to be measured, and common to both are conducted [(r) to (q)], and based on the results, comparative analysis on peptide/protein of the sample to be measured (t). Without regarding an ion derived from all components of the sample to be measured as precursor ion, MS/MS spectrum is obtained and identification on a plurality of components of the sample to be measured can be conducted for a short time and with high sensitivity.

摘要翻译： 本发明实现了能够以高速，高灵敏度鉴定蛋白质或肽的质量分析方法。 从健康人的标准样品获得质谱，从质谱中选择离子作为前体离子，得到前体离子的质谱[（a）〜（d）]。 从要测量的患者的感兴趣样品获得质谱，从作为前体离子的质谱中选择标准样品的前体离子以外的离子，得到前体离子的质谱[（ g）至（k）]。 对标准样品和待测样品的肽/蛋白质的特征进行识别，并且两者共同进行[（r）至（q）]，并且基于结果，将样品的肽/蛋白质进行比较分析 测量（t）。 不考虑作为前体离子测定的样品的所有成分的离子，可以获得MS / MS谱，并且可以在短时间内以高灵敏度进行被测定样品的多个成分的鉴定。