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公开(公告)号：US5788758A
公开(公告)日：1998-08-04
申请号：US874409
申请日：1997-06-13
申请人： Hiroshi Sawada , Shinsuke Imai , Muneaki Tomotake , Kaori Akita
发明人： Hiroshi Sawada , Shinsuke Imai , Muneaki Tomotake , Kaori Akita
CPC分类号： A23L5/48
摘要： The present invention is intended to provide a method for manufacturing a natural blue pigment that can be used in food products and the like, and the present invention relates to a method for manufacturing a blue pigment, comprising the steps of combining and warming the reaction product of PeCSO and alliinase, an amino acid, and allicin in an aqueous solution. Other embodiments of the present invention include the aforementioned method for manufacturing a blue pigment, further comprising the steps of extracting the reaction product of PeCSO and alliinase with a water-insoluble organic solvent, and allowing the reaction product to further react with an amino acid in an aqueous solution with the resulting component, and subsequently adding allicin to the reaction mixture to develop blue color, as well as the aforementioned method for manufacturing a blue pigment, further comprising the steps of combining the reaction product of PeCSO and alliinase, an amino acid, and allicin in an aqueous solution, and subsequently heat treating these materials before they are warmed.
摘要翻译： 本发明旨在提供可用于食品等的天然蓝色颜料的制造方法，本发明涉及一种蓝色颜料的制造方法，其特征在于，包括以下步骤：将反应产物 的PeCSO和蒜氨酸酶，氨基酸和大蒜素在水溶液中。 本发明的其它实施方案包括上述蓝色颜料的制造方法，其还包括以下步骤：用水不溶性有机溶剂萃取PeCSO和蒜氨酸酶的反应产物，并使反应产物进一步与 与所得组分的水溶液，然后将大蒜素加入到反应混合物中以形成蓝色，以及上述制备蓝色颜料的方法，还包括以下步骤：将PeCSO和蒜氨酸酶的反应产物，氨基酸 ，和大蒜素在水溶液中，然后在加热之前对这些材料进行热处理。
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公开(公告)号：US20050176126A1
公开(公告)日：2005-08-11
申请号：US10344158
申请日：2001-08-30
申请人： Shinsuke Imai , Nobuaki Tsuge , Muneaki Tomotake
发明人： Shinsuke Imai , Nobuaki Tsuge , Muneaki Tomotake
IPC分类号： C07K14/415 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/88 , C12N9/90 , C12N15/61 , C12N9/64 , C07H21/04
CPC分类号： C12N9/90 , C07K14/415 , C12N9/88
摘要： It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.
摘要翻译： 本发明的一个目的是提供产生泪液因子的酶的同工酶，这些同工酶的氨基酸序列和编码这些氨基酸序列的基因，本发明涉及三种类型的变应原因子的同功酶 产生有助于产生存在于洋葱等中的变应原因子的酶，由构成这些同功酶的蛋白质或多肽的SEQ ID NO.1至3表示的氨基酸序列，SEQ ID No.4所示的DNA 和5，其包含编码上述蛋白质或多肽的碱基序列，产生上述同功酶的方法，含有上述DNA的重组载体，通过用上述重组载体转化宿主细胞形成的转化体，制备 蛋白质或多肽，其具有通过培养上述宿主c产生酶活性的lachrymatory因子 和具有与对应于上述DNA的mRNA互补的碱基序列的反义RNA。
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3.发明申请公开(公告)号：US20090081754A1
公开(公告)日：2009-03-26
申请号：US12051023
申请日：2008-03-19
申请人： Shinsuke IMAI , Nobuaki Tsuge , Muneaki Tomotake
发明人： Shinsuke IMAI , Nobuaki Tsuge , Muneaki Tomotake
CPC分类号： C12N9/90 , C07K14/415 , C12N9/88
摘要： It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.
摘要翻译： 本发明的一个目的是提供产生泪液因子的酶的同工酶，这些同工酶的氨基酸序列和编码这些氨基酸序列的基因，本发明涉及三种类型的变应原因子的同功酶 产生有助于产生存在于洋葱等中的变应原因子的酶，由构成这些同功酶的蛋白质或多肽的SEQ ID NO.1至3表示的氨基酸序列，SEQ ID No.4所示的DNA 和5，其包含编码上述蛋白质或多肽的碱基序列，产生上述同功酶的方法，含有上述DNA的重组载体，通过用上述重组载体转化宿主细胞形成的转化体，制备 蛋白质或多肽，其具有通过培养上述宿主c产生酶活性的lachrymatory因子 和具有与对应于上述DNA的mRNA互补的碱基序列的反义RNA。
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公开(公告)号：US07371554B2
公开(公告)日：2008-05-13
申请号：US10344158
申请日：2001-08-30
申请人： Shinsuke Imai , Nobuaki Tsuge , Muneaki Tomotake
发明人： Shinsuke Imai , Nobuaki Tsuge , Muneaki Tomotake
CPC分类号： C12N9/90 , C07K14/415 , C12N9/88
摘要： It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.
摘要翻译： 本发明的一个目的是提供产生泪液因子的酶的同工酶，这些同工酶的氨基酸序列和编码这些氨基酸序列的基因，本发明涉及三种类型的变应原因子的同功酶 产生有助于产生存在于洋葱等中的变应原因子的酶，由构成这些同功酶的蛋白质或多肽的SEQ ID NO.1至3表示的氨基酸序列，SEQ ID No.4所示的DNA 和5，其包含编码上述蛋白质或多肽的碱基序列，产生上述同功酶的方法，含有上述DNA的重组载体，通过用上述重组载体转化宿主细胞形成的转化体，制备 蛋白质或多肽，其具有通过培养上述宿主c产生酶活性的lachrymatory因子 和具有与对应于上述DNA的mRNA互补的碱基序列的反义RNA。
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