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公开(公告)号：US5464516A
公开(公告)日：1995-11-07
申请号：US235934
申请日：1994-05-02
申请人： Hisao Takeda , Youichi Koshiji , Hidehiro Kubota , Takashi Iizuka
发明人： Hisao Takeda , Youichi Koshiji , Hidehiro Kubota , Takashi Iizuka
CPC分类号： G01N27/44747 , B01D57/02 , Y10T428/254 , Y10T428/31725
摘要： An electrophoresis separation layer is produced which comprises a polyacrylamide gel which remains stable even when stored for a long time and is available for analyzing substances of an extremely wide molecular weight range in electrophoresis for determining molecular weight. The electrophoresis separation layer of the present invention includes a polyacrylamide gel with an aqueous solution containing an amine of a specific pH value, an ampholyte and an acid at a specific ratio as an electrolytic solution in the gel. This separation layer can be produced by lowering the pH value of the electrolytic solution in the polyacrylamide gel to such an extent as not to cause any hydrolysis of amide while achieving a suitable potential gradient distribution in the gel during the electrophoresis.
摘要翻译： 生成电泳分离层，其包含聚丙烯酰胺凝胶，即使长时间保存也保持稳定，并且可用于分析用于确定分子量的电泳中极宽分子量范围的物质。 本发明的电泳分离层包括具有特定pH值的胺的水溶液的聚丙烯酰胺凝胶，作为凝胶中的电解液的特定比例的两性电解质和酸。 该分离层可以通过将聚丙烯酰胺凝胶中的电解液的pH值降低到不会引起酰胺的水解，同时在电泳期间在凝胶中达到合适的电位梯度分布的程度来制备。
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2.发明授权
公开(公告)号：US4319883A
公开(公告)日：1982-03-16
申请号：US133977
申请日：1980-03-26
CPC分类号： G01N33/52 , Y10T436/203332
摘要： Disclosed are a method and an apparatus for determining catecholic compounds and their related compounds contained in a substance by utilizing the specific reactivity of the compounds with ferric chloride and potassium ferricyanide as reaction reagents. The reaction products are analyzed by spectrophotometry, preferably in combination with high-performance liquid chromatography, so as to determine catecholic compounds and their related compounds.
摘要翻译： 公开了通过利用化合物与氯化铁和铁氰化钾作为反应试剂的具体反应性来确定物质中所含的儿茶酚化合物及其相关化合物的方法和装置。 通过分光光度法分析反应产物，优选与高效液相色谱法组合，以确定儿茶酚化合物及其相关化合物。
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