公开(公告)号：US20250136639A1

公开(公告)日：2025-05-01

申请号：US18645245

申请日：2024-04-24

Applicant: Hoffmann-La Roche Inc.

Inventor： ROBERTO FALKENSTEIN ,  BERNHARD SPENSBERGER

IPC: C07K1/18 ,  B01D15/36 ,  C07K14/505

Abstract: Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange Toyopearl® SP-650 chromatography material and employing a second wash step with an increased pH value compared to the first wash step a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield and suitability for large scale applications.

公开(公告)号：US20210155655A1

公开(公告)日：2021-05-27

申请号：US17145653

申请日：2021-01-11

Applicant: Hoffmann-La Roche Inc.

Inventor： ROBERTO FALKENSTEIN ,  BERNHARD SPENSBERGER

IPC: C07K1/18 ,  B01D15/36 ,  C07K14/505

Abstract: Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange Toyopearl® SP-650 chromatography material and employing a second wash step with an increased pH value compared to the first wash step a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield and suitability for large scale applications.

公开(公告)号：US20190300567A1

公开(公告)日：2019-10-03

申请号：US16245081

申请日：2019-01-10

Applicant: Hoffmann-La Roche Inc.

Inventor： ROBERTO FALKENSTEIN ,  BERNHARD SPENSBERGER

IPC: C07K1/18 ,  C07K14/505 ,  B01D15/36

Abstract: Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange Toyopearl® SP-650 chromatography material and employing a second wash step with an increased pH value compared to the first wash step a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield and suitability for large scale applications.