公开(公告)号：US20210003559A1

公开(公告)日：2021-01-07

申请号：US16912391

申请日：2020-06-25

申请人： Hoffmann-La Roche Inc. ,  F. Hoffmann-La Roche AG ,  Emulate, Inc.

发明人： Cristina Bertinetti-Lapatki ,  Lauriane Cabon ,  Adrian B. Roth ,  Annie Moisan ,  Nikolche Gjorevski ,  Jordan S. Kerns ,  Geraldine A. Hamilton ,  Catherine Karalis ,  Heather Grant ,  Riccardo Barrile ,  Debora Barreiros Petropolis ,  Chaitra Belgur

IPC分类号： G01N33/50

摘要： Disclosed herein is a method for assessing a compound interacting with a target on polarized epithelial cells. The method comprising the steps of providing an organ chip comprising a main channel and polarized epithelial cells, wherein the main channel is divided into an apical channel and a basal channel separated by the polarized epithelial cells, wherein the apical side of the polarized epithelial cells is directed towards the apical channel and the basolateral side of the polarized epithelial cells is directed towards the basal channel. Determining the localization and optionally the expression level of the target on the polarized epithelial cells. Administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the basal channel, when the target is localized on the basolateral side of the epithelial cells or administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the apical channel, when the target is localized on the apical side of the epithelial cells. Measuring a parameter of the administration of the compound and the peripheral blood mononuclear cells.