公开(公告)号：US20070218553A1

公开(公告)日：2007-09-20

申请号：US11748735

申请日：2007-05-15

申请人： Horacio RODRIGUEZ RILO ,  Arthur Helmicki ,  William Heineman ,  H. Halsall ,  Kevin Schlueter ,  Anne Maghasi

发明人： Horacio RODRIGUEZ RILO ,  Arthur Helmicki ,  William Heineman ,  H. Halsall ,  Kevin Schlueter ,  Anne Maghasi

IPC分类号： C12N5/06

CPC分类号： C12M47/04 ,  C12M29/18 ,  C12M41/48

摘要： An apparatus 20 for the separation of a subpopulation of cells from an intact organ or other biological material is provided. The apparatus 20 includes: (1) a digestion chamber 24 that integrates the primary digestion process, (2) a measuring cylinder 26, (3) a cell collection chamber 28, (4) a heat exchanger 30 for raising and lowering temperatures in the digestion chamber 24 to activate or inactivate enzymes, (5) sensors 112, 114, 116, 118, 120, 122 to complete a closed feedback loop for allowing optimization of the digestion process, and (6) mock cells which mimic the cells to be harvested and which are used to fully optimize the process without unnecessary destruction of harvested cells. The manipulation of the digestion process may be manual or may be automated under computer control.

摘要翻译： 提供了用于从完整的器官或其他生物材料分离细胞亚群的装置20。 装置20包括：（1）集成了初级消化处理的消化室24，（2）测量筒26，（3）细胞收集室28，（4）用于升高和降低温度的热交换器30 消化室24以激活或灭活酶，（5）传感器112,114,116,118,120,122以完成用于允许消化过程优化的闭合反馈回路，和（6）模拟细胞的模拟细胞 收获，并且其用于完全优化该过程，而不会不必要地破坏收获的细胞。 消化过程的操作可以是手动的或者可以在计算机控制下自动化。