公开(公告)号：US20240302361A1

公开(公告)日：2024-09-12

申请号：US18573392

申请日：2022-06-23

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Abdon SEPULVEDA ,  Victor ESTRADA ,  Ruoda ZHENG ,  Yilian WANG

IPC: G01N33/543 ,  B01L3/00 ,  C12M1/00 ,  C12M3/06 ,  G01N33/569

CPC classification number: G01N33/54333 ,  B01L3/502761 ,  C12M23/16 ,  C12M47/04 ,  G01N33/569 ,  B01L2200/0652 ,  B01L2200/16 ,  B01L2400/043

Abstract: The present embodiments relate generally to a method and apparatus of capturing and releasing magnetically activated cells. Embodiments include a microfluidic device capable of capturing a large array of magnetically functionalized cells and releasing any one specific captured cell without releasing the rest of the cells. Methods and devices according to embodiments provide an improvement over current microfluidic cell sorting approaches due to the use of a simple mechanism, which simultaneously allows for high selectivity, and high throughput potential.

公开(公告)号：US12071611B2

公开(公告)日：2024-08-27

申请号：US17255381

申请日：2019-07-03

Applicant: Chengdu Precisome Biotechnology Co., Ltd. ,  Qiang Xu

Inventor： Hubing Shi ,  Yanchu Li ,  Qi Xu ,  Xiangju Kong ,  Xueyan Wang ,  Hewen Han

IPC: B01L3/00 ,  A61M1/36 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B23Q17/24 ,  C12M1/00 ,  C12M1/34 ,  C12N5/09 ,  C12Q1/18 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N33/50 ,  G01N33/68

CPC classification number: C12M47/04 ,  A61M1/3679 ,  C12N5/0693 ,  A61M2205/0244 ,  C12N2509/00

Abstract: Disclosed are a rare cell capture system and an application thereof. The system comprises a fluid tube device, a circulation power apparatus device, a component capture device and an optional anticoagulant release device, the circulation power apparatus device and the component capture device being connected in series to a fluid circulation system via the fluid tube device to form an extracorporeal fluid circulation pathway, the component capture device comprising a microfluidic chip or a chipset. Also disclosed is a method for using the capture system to capture rare cells in blood. The system and the application thereof have the advantages of being large-capacity, in-line and low-hemolysis.

公开(公告)号：US12044614B2

公开(公告)日：2024-07-23

申请号：US17589507

申请日：2022-01-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/1433 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  G01N15/00 ,  G01N15/01 ,  G01N15/10

CPC classification number: G01N15/1484 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1433 ,  G01N35/00029 ,  G01N35/1011 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/021 ,  B01L3/502746 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00158 ,  G01N2035/1039

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US12018239B2

公开(公告)日：2024-06-25

申请号：US17103539

申请日：2020-11-24

Applicant: KATAOKA CORPORATION ,  NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Masami Suzuki ,  Norio Nishi ,  Junichi Matsumoto ,  Kimio Sumaru ,  Toshiyuki Kanamori

IPC: C12M1/34 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  C12M1/32 ,  C12M1/42 ,  C12N1/00 ,  C12N1/02 ,  C12N5/00 ,  C12N13/00

CPC classification number: C12M1/42 ,  C12M1/005 ,  C12M1/3446 ,  C12M23/20 ,  C12M25/06 ,  C12M31/02 ,  C12M33/06 ,  C12M33/10 ,  C12M47/04 ,  C12N1/00 ,  C12N1/02 ,  C12N5/0081 ,  C12N13/00

Abstract: A laser processing machine for killing specific cells from a group of cells on a surface of a layer containing an ingredient capable of absorbing laser light, the laser processing machine being configured to: control a laser light source to output laser light at 5W or less and at a wavelength of 380 nm or greater such that the laser light source is applied to a second area on a second surface of the layer opposed to the first surface; and control an actuator to provide a relative movement between the second area where the laser light is applied and the layer at a rate of 2000 mm/sec or less such that the irradiated second area absorbs energy to generate heat that kills unwanted cells on a first area of the first surface and the laser light does not instantly kill the specific cells on the first area upon irradiation.

公开(公告)号：US20240200012A1

公开(公告)日：2024-06-20

申请号：US18410607

申请日：2024-01-11

Applicant: Global Life Sciences Solutions USA LLC

Inventor： Weston Blaine Griffin ,  Alex D Corwin ,  Xiaohua Zhang ,  Reginald Donovan Smith ,  Zhen Liu ,  Chengkun Zhang ,  Vandana Keskar ,  Brian Michael Davis ,  Kashan Shaikh

IPC: C12M1/26 ,  C12M1/00 ,  C12M1/04 ,  C12M1/12 ,  C12M1/34

CPC classification number: C12M33/14 ,  C12M23/24 ,  C12M25/02 ,  C12M29/10 ,  C12M41/44 ,  C12M47/04

Abstract: A bioprocessing system includes a first module configured for enriching and isolating a population of cells, a second module configured for activating, genetically modifying, and expanding the population of cells, and a third module configured for harvesting the expanded population of cells.

公开(公告)号：US20240191203A1

公开(公告)日：2024-06-13

申请号：US18344024

申请日：2023-06-29

Applicant: New York Stem Cell Foundation, Inc.

Inventor： Scott Noggle ,  Kevin C. Eggan ,  Stephen Chang ,  Susan L. Solomon

IPC: C12N5/074 ,  C12M1/00 ,  C12M1/12 ,  C12M1/42

CPC classification number: C12N5/0696 ,  C12M23/04 ,  C12M35/02 ,  C12M35/08 ,  C12M47/04

Abstract: The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells.

公开(公告)号：US11946855B2

公开(公告)日：2024-04-02

申请号：US18195084

申请日：2023-05-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/10 ,  G01N33/487 ,  G06V20/69 ,  G01N15/02 ,  G01N35/00

CPC classification number: G01N15/1484 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00158 ,  G06V20/69

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US11946853B2

公开(公告)日：2024-04-02

申请号：US17396530

申请日：2021-08-06

Applicant: cytena GmbH

Inventor： Jonas Schoendube ,  Andre Gross

IPC: C12M1/36 ,  B01L3/02 ,  C12M1/00 ,  C12M1/34 ,  G01N15/10 ,  G01N15/14 ,  G01N35/10

CPC classification number: G01N15/1463 ,  B01L3/02 ,  C12M1/36 ,  C12M41/36 ,  C12M47/04 ,  G01N15/10 ,  G01N15/1427 ,  G01N15/1468 ,  B01L2200/025 ,  B01L2300/0654 ,  B01L2300/0858 ,  B01L2300/168 ,  G01N2015/1006 ,  G01N2035/1034

Abstract: A apparatus for detecting cells or particles in a fluid container includes a dispenser configured to dispense at least one cell or at least one particle into a defined sub-volume of a fluid with which the fluid container is at least partially filled, and a detection apparatus configured to, in a time-coordinated manner with dispensing the at least one cell or the at least one particle by the dispenser, perform a detection in the defined sub-volume and/or in one or several sub-volumes underneath the defined sub-volume in order to sense the at least one cell or the at least one particle when entering the fluid or immediately after entering the fluid.

公开(公告)号：US11905509B2

公开(公告)日：2024-02-20

申请号：US17512558

申请日：2021-10-27

Applicant: Koligo Therapeutics, Inc

Inventor： Rolf Wolters ,  Jules Valenti ,  Tom Gurski

IPC: C12M1/00 ,  A61K35/12 ,  B04B1/02 ,  C12M1/26 ,  B04B1/00

CPC classification number: C12M47/04 ,  A61K35/12 ,  B04B1/00 ,  B04B1/02 ,  C12M33/10 ,  C12M45/05 ,  C12M45/06 ,  C12M45/09

Abstract: Cell separation systems and methods of separating cells are disclosed. In an embodiment, a cell separation system is described that comprises a non-transitory storage device that executes a centrifugation program to separate cell volume from biologic material volume; a heating mechanism; a containment mechanism; and an assembly comprised of a single-walled centrifugation bowl. In an embodiment, methods of separating cells are disclosed whereby cells are separated by agitating a volume of biologic material and a volume digestion media to form a digested volume of biologic material; centrifuging the digested volume of biologic material; removing a portion of a resulting waste via at least one fluid outlet; isolating a different portion of the waste, and removing the concentrated cell volumes from the reservoir.

公开(公告)号：US20240052292A1

公开(公告)日：2024-02-15

申请号：US18447437

申请日：2023-08-10

Applicant: CANON MEDICAL SYSTEMS CORPORATION ,  Canon Kabushiki Kaisha

Inventor： Mayumi SAKAGAMI ,  Koji HIRATA

IPC: C12M1/00 ,  C12M1/36

CPC classification number: C12M47/04 ,  C12M41/48

Abstract: A cell management system according to an embodiment includes an analysis apparatus and a determination apparatus. The analysis apparatus acquires a first identification code from a processing sample from which a cell used for treatment is produced, or from a processed product based on the processing sample, and acquires a second identification code from a reference sample. Based on the first identification code and the second identification code, the determination apparatus determines the consistency of a subject from whom the processing sample is obtained and a subject from whom the reference sample is obtained.