公开(公告)号：US5753445A

公开(公告)日：1998-05-19

申请号：US233390

申请日：1994-04-26

申请人： Howard M. Fillit ,  Peter C. Harpel

发明人： Howard M. Fillit ,  Peter C. Harpel

IPC分类号： G01N33/86 ,  G01N33/53

CPC分类号： G01N33/86 ,  G01N2333/8128 ,  G01N2400/40 ,  Y10S436/821

摘要： This invention pertains to a method of detecting, in a sample obtained from an individual, anti-heparin antibodies which inhibit the formation of the heparin accelerated antithrombin III-thrombin complex. In the present method, the presence of such anti-heparin antibodies are detected directly (by detecting the presence of anti-heparin antibodies themselves) or indirectly (by detecting the presence or formation of the heparin accelerated antithrombin III-thrombin complex). In one embodiment of the present method, antibodies which react with or interfere with the heparin pentasaccharide which binds antithrombin III in such a manner that binding to antithrombin III is inhibited are detected. In a specific embodiment of the present method, the anti-heparin antibody detected is one which reacts with or interferes with the disaccharide UA-2S/GlcNs-6 present in residues IV and V of the heparin pentasaccharide that binds antithrombin III.

摘要翻译： 本发明涉及从个体获得的样品中检测抑制肝素加速抗凝血酶III-凝血酶复合物形成的抗肝素抗体的方法。 在本方法中，直接检测这种抗肝素抗体的存在（通过检测本身存在抗肝素抗体）或间接检测（通过检测肝素加速的抗凝血酶III-凝血酶复合物的存在或形成）。 在本方法的一个实施方案中，检测了以与抗凝血酶III结合的方式结合抗凝血酶III的肝素五糖起反应或干扰的抗体。 在本方法的具体实施方案中，检测的抗肝素抗体是与存在于结合抗凝血酶III的肝素五糖的残基IV和V中的二糖UA-2S / GlcNs-6反应或干扰的抗肝素抗体。