公开(公告)号：US20160219844A1

公开(公告)日：2016-08-04

申请号：US15021026

申请日：2014-09-12

申请人： ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI

发明人： Brian BROWN ,  Judith AGUDO

IPC分类号： A01K67/027 ,  G01N33/566 ,  C07K14/725

CPC分类号： A01K67/0275 ,  A01K2217/30 ,  A01K2227/105 ,  A01K2267/0387 ,  A01K2267/0393 ,  C07K14/7051 ,  C12N15/8509 ,  G01N33/566 ,  G01N2333/70503

摘要： This invention relates to a transgenic non-human mammal whose genome comprises a polynucleotide sequence encoding a T cell receptor that is specific to a fluorescent protein, where the T cell of the non-human mammal comprises the T cell receptor. The present invention also relates to an isolated T cell from the transgenic non-human mammal of the present invention, an isolated T cell comprising an expression construct comprising a polynucleotide sequence that encodes a T cell receptor that is specific to a fluorescent protein, methods of making transgenic non-human mammals comprising T cell receptors that are specific to a fluorescent protein, a method of depleting cells in a non-human mammal using isolated T cells that encode a T cell receptor that is specific to a target protein, and a method of characterizing a T cell response to an agent.

摘要翻译： 本发明涉及一种转基因非人哺乳动物，其基因组包含编码对荧光蛋白特异性的T细胞受体的多核苷酸序列，其中非人哺乳动物的T细胞包含T细胞受体。 本发明还涉及本发明的转基因非人哺乳动物的分离的T细胞，包含含有编码对荧光蛋白特异的T细胞受体的多核苷酸序列的表达构建体的分离的T细胞， 制备包含对荧光蛋白特异性的T细胞受体的转基因非人哺乳动物，使用编码靶蛋白特异性的T细胞受体的分离的T细胞消耗非人哺乳动物细胞的方法，以及方法 表征T细胞对药剂的反应。