公开(公告)号：US09249402B2

公开(公告)日：2016-02-02

申请号：US14350827

申请日：2012-09-26

Applicant: IFP ENERGIES NOUVELLES

Inventor： Fadhel Ben Chaabane ,  Bernard Chaussepied

IPC: C12N9/42 ,  C12P21/00 ,  C12N1/14 ,  C12N1/38 ,  C12N9/18

CPC classification number: C12N9/2437 ,  C12N1/14 ,  C12N1/38 ,  C12N9/18 ,  C12P21/00 ,  C12Y301/01073 ,  Y02E50/16

Abstract: A process for the production of cellulases and hemicellulases using a strain of a filamentous fungus in a stirred and aerated bioreactor, in at least two phases, a phase a) for growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, a phase b) for the continuous production of cellulases, in which at least one carbonaceous inducer substrate is supplied at a supply rate which is constant over a period of at least more than 200 h, said carbonaceous substrate being at least one aqueous hemicellulosic hydrolysate solution obtained from an acid pre-treatment of a lignocellulosic substrate, said aqueous hemicellulosic hydrolysate solution not undergoing prior sterilization and not undergoing pH rectification, being operated at a dilution rate in the range 0.001 to 0.02 h−1.

Abstract translation: 一种在搅拌和曝气的生物反应器中在至少两个阶段中使用丝状真菌菌株生产纤维素酶和半纤维素酶的方法，a）用于在至少一个含碳生长底物存在下生长所述菌株的相a） 封闭反应器，b）用于连续生产纤维素酶，其中至少一种含碳诱导剂底物以至少大于200小时的时间内恒定的供应速率供应，所述碳质底物是至少一种含水的 从木质纤维素底物的酸预处理得到的半纤维素水解物溶液，所述水解半纤维素水解物溶液不经历先前灭菌并且不经历pH精馏，以0.001至0.02h -1的稀释速率操作。

公开(公告)号：US10214734B2

公开(公告)日：2019-02-26

申请号：US15500299

申请日：2015-07-27

Applicant: IFP Energies nouvelles

Inventor： Fadhel Ben Chaabane ,  Etienne Jourdier ,  Celine Cohen ,  Bernard Chaussepied

IPC: C12N1/06 ,  C12N1/14 ,  C12N9/24 ,  C12N9/42

Abstract: The invention relates to a process for the production of an enzymatic cocktail from a cellulolytic microorganism producing cellulases and/or hemi-cellulases, comprising: a step for the production of enzymes with a medium being obtained containing enzymes and a microorganism must, said must is separated, or not separated, from the liquid containing said enzymes, a step of cooling said must to a temperature below the temperature of the enzyme production step and for a time such that the beta-glucosidase concentration of the liquid originating from the cooling step is greater than that of the liquid originating from the enzyme production step, and/or the solid volume/total volume ratio is less than said ratio for the enzyme production step, and an enzymatic cocktail is obtained at the end of the cooling step.

公开(公告)号：US20140295524A1

公开(公告)日：2014-10-02

申请号：US14350827

申请日：2012-09-26

Applicant: IFP ENERGIES NOUVELLES

Inventor： Fadhel Ben Chaabane ,  Bernard Chaussepied

IPC: C12N9/42

CPC classification number: C12N9/2437 ,  C12N1/14 ,  C12N1/38 ,  C12N9/18 ,  C12P21/00 ,  C12Y301/01073 ,  Y02E50/16

Abstract: A process for the production of cellulases and hemicellulases using a strain of a filamentous fungus in a stirred and aerated bioreactor, in at least two phases, a phase a) for growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, a phase b) for the continuous production of cellulases, in which at least one carbonaceous inducer substrate is supplied at a supply rate which is constant over a period of at least more than 200 h, said carbonaceous substrate being at least one aqueous hemicellulosic hydrolysate solution obtained from an acid pre-treatment of a lignocellulosic substrate, said aqueous hemicellulosic hydrolysate solution not undergoing prior sterilization and not undergoing pH rectification, being operated at a dilution rate in the range 0.001 to 0.02 h−1.

Abstract translation: 一种在搅拌和曝气的生物反应器中在至少两个阶段中使用丝状真菌菌株生产纤维素酶和半纤维素酶的方法，a）用于在至少一个含碳生长底物存在下生长所述菌株的相a） 封闭反应器，b）用于连续生产纤维素酶，其中至少一种含碳诱导剂底物以至少大于200小时的时间内恒定的供应速率供应，所述碳质底物是至少一种含水的 从木质纤维素底物的酸预处理得到的半纤维素水解物溶液，所述水解半纤维素水解物溶液不经历先前灭菌并且不经历pH精馏，以0.001至0.02h -1的稀释速率操作。