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    USE OF CO2 TO DEACTIVATE A CELLULOLYTIC MICROORGANISM USED IN THE BIOCHEMICAL CONVERSION OF LIGNOCELLULOSIC MATERIALS
    3.
    发明申请
    USE OF CO2 TO DEACTIVATE A CELLULOLYTIC MICROORGANISM USED IN THE BIOCHEMICAL CONVERSION OF LIGNOCELLULOSIC MATERIALS 审中-公开
    使用二氧化碳消除细菌生物化学转化中使用的纤维素微生物

    公开(公告)号：US20150152400A1

    公开(公告)日：2015-06-04

    申请号：US14408612

    申请日：2013-06-10

    Applicant: IFP ENERGIES NOUVELLES

    Inventor： Fadhel Ben Chaabane ,  Sylvain Louret

    IPC: C12N9/24

    CPC classification number: C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12P21/00 ,  C12P2203/00 ,  C12Y302/01004 ,  Y02E50/16

    Abstract: A process for the production of an enzymatic cocktail with a cellulolytic microorganism comprises two phases:a phase a) for growth of said microorganism in a closed reactor in the presence of a carbonaceous growth solution;a phase b) for the production of said enzymatic cocktail carried out with a supply of carbonaceous production solution the concentration of carbonaceous substrate of which is in the range 150 to 400 g/L, said carbonaceous production solution comprising a carbonaceous inducer substrate;characterized in that said carbonaceous inducer substrate is a liquid residue obtained from a step for pre-treatment of lignocellulosic materials, the C5 sugar oligomers of which represent at least 1% by weight of the total sugars present in said liquid residue, and at least 0.3% by weight of the total sugars present in said carbonaceous production solution.

    Abstract translation: 用纤维素分解微生物生产酶促混合物的方法包括两个阶段：一个阶段a）用于在碳质生长溶液存在下在封闭反应器中生长所述微生物; a）阶段b），用于生产所述酶促混合物，其中碳质生物溶液的碳浓度为150-400g / L，所述含碳生产溶液包含碳质诱导剂底物; 其特征在于，所述含碳诱导剂底物是由用于预处理木质纤维素材料的步骤获得的液体残余物，所述木质纤维素材料的C5糖低聚物占所述液体残余物中存在的总糖的至少1重量％，并且至少0.3 占所述碳质生产溶液中存在的总糖的重量％。

    PROCESS FOR THE CONTINUOUS PRODUCTION OF CELLULASES BY A FILAMENTOUS FUNGUS USING A CARBON SUBSTRATE OBTAINED FROM AN ACID PRETREATMENT
    4.
    发明申请
    PROCESS FOR THE CONTINUOUS PRODUCTION OF CELLULASES BY A FILAMENTOUS FUNGUS USING A CARBON SUBSTRATE OBTAINED FROM AN ACID PRETREATMENT 有权
    通过使用从酸性预处理获得的碳质基底的真菌真菌连续生产细胞的方法

    公开(公告)号：US20140295524A1

    公开(公告)日：2014-10-02

    申请号：US14350827

    申请日：2012-09-26

    Applicant: IFP ENERGIES NOUVELLES

    Inventor： Fadhel Ben Chaabane ,  Bernard Chaussepied

    IPC: C12N9/42

    CPC classification number: C12N9/2437 ,  C12N1/14 ,  C12N1/38 ,  C12N9/18 ,  C12P21/00 ,  C12Y301/01073 ,  Y02E50/16

    Abstract: A process for the production of cellulases and hemicellulases using a strain of a filamentous fungus in a stirred and aerated bioreactor, in at least two phases, a phase a) for growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, a phase b) for the continuous production of cellulases, in which at least one carbonaceous inducer substrate is supplied at a supply rate which is constant over a period of at least more than 200 h, said carbonaceous substrate being at least one aqueous hemicellulosic hydrolysate solution obtained from an acid pre-treatment of a lignocellulosic substrate, said aqueous hemicellulosic hydrolysate solution not undergoing prior sterilization and not undergoing pH rectification, being operated at a dilution rate in the range 0.001 to 0.02 h−1.

    Abstract translation: 一种在搅拌和曝气的生物反应器中在至少两个阶段中使用丝状真菌菌株生产纤维素酶和半纤维素酶的方法，a）用于在至少一个含碳生长底物存在下生长所述菌株的相a） 封闭反应器，b）用于连续生产纤维素酶，其中至少一种含碳诱导剂底物以至少大于200小时的时间内恒定的供应速率供应，所述碳质底物是至少一种含水的 从木质纤维素底物的酸预处理得到的半纤维素水解物溶液，所述水解半纤维素水解物溶液不经历先前灭菌并且不经历pH精馏，以0.001至0.02h -1的稀释速率操作。

    Method for producing an enzymatic cocktail from fungal must
    5.
    发明授权
    Method for producing an enzymatic cocktail from fungal must 有权

    公开(公告)号：US10214734B2

    公开(公告)日：2019-02-26

    申请号：US15500299

    申请日：2015-07-27

    Applicant: IFP Energies nouvelles

    Inventor： Fadhel Ben Chaabane ,  Etienne Jourdier ,  Celine Cohen ,  Bernard Chaussepied

    IPC: C12N1/06 ,  C12N1/14 ,  C12N9/24 ,  C12N9/42

    Abstract: The invention relates to a process for the production of an enzymatic cocktail from a cellulolytic microorganism producing cellulases and/or hemi-cellulases, comprising: a step for the production of enzymes with a medium being obtained containing enzymes and a microorganism must, said must is separated, or not separated, from the liquid containing said enzymes, a step of cooling said must to a temperature below the temperature of the enzyme production step and for a time such that the beta-glucosidase concentration of the liquid originating from the cooling step is greater than that of the liquid originating from the enzyme production step, and/or the solid volume/total volume ratio is less than said ratio for the enzyme production step, and an enzymatic cocktail is obtained at the end of the cooling step.

    PROCESS FOR THE PRODUCTION OF AN ENZYMATIC COCKTAIL USING SOLID RESIDUES FROM A PROCESS FOR THE BIOCHEMICAL COVERSION OF LIGNOCELLULOSIC MATERIALS
    7.
    发明申请
    PROCESS FOR THE PRODUCTION OF AN ENZYMATIC COCKTAIL USING SOLID RESIDUES FROM A PROCESS FOR THE BIOCHEMICAL COVERSION OF LIGNOCELLULOSIC MATERIALS 有权
    使用固体残留物生产化学纤维素生物化学方法的方法

    公开(公告)号：US20140349373A1

    公开(公告)日：2014-11-27

    申请号：US14365274

    申请日：2012-11-27

    Applicant: IFP Energies nouvelles

    Inventor： Fadhel Ben Chaabane ,  Sylvain Louret

    IPC: C12N9/42

    CPC classification number: C12N9/2437 ,  C12N1/16 ,  C12N9/2477 ,  C12P7/10 ,  C12P21/00 ,  C12P2203/00 ,  C12Y302/01004 ,  C12Y302/01074 ,  Y02E50/16

    Abstract: The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases: a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L; a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.

    Abstract translation: 本发明涉及通过用纤维素分解微生物浸没培养生产酶促混合物的方法，该方法包括两相：一相a）用于在封闭反应器中在至少一个含碳生长基质存在下生长所述微生物，所述相 生长期以含碳生长基质浓度在10〜90g / L范围内进行; b）用于生产其中提供至少一个含碳诱导剂底物的酶混合物，所述碳质诱导剂底物是从已经经过预处理的木质纤维素材料的酶水解步骤获得的至少一种固体残余物 所述生产阶段的碳质生产基质的浓度在150至400g / L的范围内进行。

    Process for the production of an enzymatic cocktail using liquid residues from a process for the biochemical conversion of lignocellulosic materials
    10.
    发明授权
    Process for the production of an enzymatic cocktail using liquid residues from a process for the biochemical conversion of lignocellulosic materials 有权

    公开(公告)号：US10030236B2

    公开(公告)日：2018-07-24

    申请号：US14408612

    申请日：2013-06-10

    Applicant: IFP ENERGIES NOUVELLES

    Inventor： Fadhel Ben Chaabane ,  Sylvain Louret

    IPC: C12N9/24 ,  C12P21/00 ,  C12N9/42

    Abstract: A process for the production of an enzymatic cocktail with a cellulolytic microorganism comprises two phases: a phase a) for growth of said microorganism in a closed reactor in the presence of a carbonaceous growth solution; a phase b) for the production of said enzymatic cocktail carried out with a supply of carbonaceous production solution the concentration of carbonaceous substrate of which is in the range 150 to 400 g/L, said carbonaceous production solution comprising a carbonaceous inducer substrate; characterized in that said carbonaceous inducer substrate is a liquid residue obtained from a step for pre-treatment of lignocellulosic materials, the C5 sugar oligomers of which represent at least 1% by weight of the total sugars present in said liquid residue, and at least 0.3% by weight of the total sugars present in said carbonaceous production solution.

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