公开(公告)号：US20170211051A1

公开(公告)日：2017-07-27

申请号：US15500299

申请日：2015-07-27

Applicant: IFP Energies nouvelles

Inventor： Fadhel BEN CHAABANE ,  Etienne JOURDIER ,  Celine COHEN ,  Bernard CHAUSSEPIED

IPC: C12N9/42 ,  C12N9/24

CPC classification number: C12N9/2445 ,  C12N1/06 ,  C12N1/14 ,  C12N9/2437 ,  C12N9/248 ,  C12Y302/01021 ,  C12Y302/01037 ,  Y02E50/16

Abstract: The invention relates to a process for the production of an enzymatic cocktail from a cellulolytic microorganism producing cellulases and/or hemi-cellulases, comprising: a step for the production of enzymes with a medium being obtained containing enzymes and a microorganism must, said must is separated, or not separated, from the liquid containing said enzymes, a step of cooling said must to a temperature below the temperature of the enzyme production step and for a time such that the beta-glucosidase concentration of the liquid originating from the cooling step is greater than that of the liquid originating from the enzyme production step, and/or the solid volume/total volume ratio is less than said ratio for the enzyme production step, and an enzymatic cocktail is obtained at the end of the cooling step.

公开(公告)号：US20210284982A1

公开(公告)日：2021-09-16

申请号：US17275703

申请日：2019-08-26

Applicant: IFP Energies Nouvelles

Inventor： Mohamed Fadhel BEN CHAABANE ,  Celine COHEN ,  Bernard CHAUSSEPIED ,  Frederic AUGIER

IPC: C12N9/42 ,  C12P7/10

Abstract: The present invention relates to a method for producing enzymes by a strain belonging to a filamentous fungus, which comprises three steps: (a) a first step of growing biomass, in the presence of at least one carbon-based growth substrate in a stirred and aerated bioreactor (1) in batch phase, (b) a second step of diluting the culture medium obtained in the first step (a), (c) a third step of producing enzymes from the diluted culture medium obtained in the second step (b), in the presence of at least one inductive carbon-based substrate, in fed-batch phase.