公开(公告)号：US20230090867A1

公开(公告)日：2023-03-23

申请号：US17947877

申请日：2022-09-19

Applicant: ILLUMINA, INC.

Inventor： Jeffrey Mandell ,  Jessica Killian

IPC: C12Q1/6869 ,  G01N33/487

Abstract: Sequencing polynucleotides using nanopores is provided herein. A polynucleotide is disposed through a nanopore's aperture such that its 3′ end is on the nanopore's first side and its 5′ end is on the nanopore's second side. On the nanopore's first side, a duplex with the polynucleotide is formed that includes a 3′ end. The duplex is extended on the first side of the nanopore by adding a nucleotide to the 3′ end of the duplex. A first force is applied disposing the 3′ end of the duplex within the aperture, and the nanopore inhibits translocation of the 3′ end of the duplex to the second side of the nanopore. A value of an electrical property of the 3′ end of the duplex and a single-stranded portion of the polynucleotide is measured. The nucleotide at the 3′ end of the duplex is identified using the measured value.