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公开(公告)号:US20220290234A1
公开(公告)日:2022-09-15
申请号:US17694404
申请日:2022-03-14
发明人: Sarah Shultzaberger , Xiaolin Wu , Eric Brustad , Niall Gormley
IPC分类号: C12Q1/6876 , C12Q1/6806 , C07H19/16
摘要: Examples provided herein are related to detecting methylcytosine and its derivatives using S-adenosyl-L-methionine analogs (xSAMs). Compositions and methods for performing such detection are disclosed. A target polynucleotide may include cytosine (C) and methylcytosine (mC). The method may include (a) protecting the C in the target polynucleotide from deamination; and (b) after step (a), deaminating the mC in the target polynucleotide to form thymine (T). Protecting the C from deamination may include adding a protective group to the 5 position of the C, e.g., using a methyltransferase enzyme that adds the first protective group from an xSAM.