公开(公告)号：US11603561B2

公开(公告)日：2023-03-14

申请号：US16766205

申请日：2018-11-30

申请人： INDIAN INSTITUTE OF TECHNOLOGY MADRAS (IIT MADRAS)

发明人： Guhan Jayaraman ,  Sudeshna Sengupta ,  Shivansh Goyal

IPC分类号： C12Q1/6851 ,  C12Q1/686

摘要： A method for direct quantification of nucleic acids in real time qPCR. The invention discloses a method for specific quantification of nucleic acids in real time qPCR. The disclosed invention can be achieved in three ways; 1) using a modified primer for qPCR quantification; 2) using strand displacement based probes for qPCR quantification; 3) using label-free endonuclease probe for qPCR quantification. The mechanism of quantification is based on the fact that, DNA, RNA or modified oligonucleotide based light-up dye-aptamer system, where dye is not fluorescent in free state but its fluorescence increases multi-fold when it binds to its specific aptamer.