公开(公告)号：US20140356917A1

公开(公告)日：2014-12-04

申请号：US14347885

申请日：2012-10-11

Applicant: INDIAN OIL CORPORATION LTD.

Inventor： Mahendra Pratap Singh ,  Manoj Kumar ,  Dheer Singh ,  Deepak Kumar Tuli ,  Ravinder Kumar Malhotra

IPC: C12P7/64 ,  C10L1/02

CPC classification number: C12P7/649 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/026 ,  C11B1/04 ,  C11B1/10 ,  C11C3/003 ,  C12N1/12 ,  C12P7/64 ,  C12R1/89 ,  Y02E50/13 ,  Y02P30/20

Abstract: The present invention provides a cost effective biotechnological process for production of bio-fuels from isolated and characterized microalgae. The algal strains used in the present invention having higher biomass, higher lipid productivity, higher pH and temperature tolerance are selected from the group consisting of Chlorella vulgaris iOC-1, Chlorella vulgaris iOC-2, Chlorella kessleri, Botrococcus bruni, Dunaliella salina and Nannochloris oculat or a combination thereof having 95-100% similarity with 18s ribosomal nucleic acids nucleotide sequences (rDNA) given for Seq. ID I, Seq. ID 2, Seq. ID 3, Seq. ID 4, Seq. ID 5 and Seq. ID 6. The present process of bio-fuel production comprises the steps of producing lipid from green algae in bioreactors by various novel steps and extracting oil from dried algal cells and ultimately producing biodiesel by transesterification of the said extracted oil.

Abstract translation: 本发明提供了用于从分离和表征的微藻生产生物燃料的成本有效的生物技术方法。 具有较高生物量，较高脂质生产力，较高pH和温度耐受性的本发明中使用的藻类菌株选自小球藻iOC-1，寻常型小球藻iOC-2，小球藻，布鲁氏菌，杜氏盐藻和Nannochloris 眼睛或其组合与具有与Seq给出的18s核糖体核酸核苷酸序列（rDNA）具有95-100％相似性。 ID I，Seq。 ID 2，Seq。 ID 3，Seq ID 4，Seq。 ID 5和Seq。 本发明的生物燃料生产方法包括以下步骤：通过各种新的步骤从生物反应器中的绿藻生产脂质，并从干藻细胞中提取油，最终通过所述提取的油的酯交换生产生物柴油。

公开(公告)号：US09441252B2

公开(公告)日：2016-09-13

申请号：US14347885

申请日：2012-10-11

Applicant: INDIAN OIL CORPORATION LTD.

Inventor： Mahendra Pratap Singh ,  Manoj Kumar ,  Dheer Singh ,  Deepak Kumar Tuli ,  Ravinder Kumar Malhotra

IPC: C12P7/64 ,  C12N1/12 ,  C10L1/02 ,  C11B1/04 ,  C11B1/10 ,  C11C3/00 ,  C12R1/89

CPC classification number: C12P7/649 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/026 ,  C11B1/04 ,  C11B1/10 ,  C11C3/003 ,  C12N1/12 ,  C12P7/64 ,  C12R1/89 ,  Y02E50/13 ,  Y02P30/20

Abstract: The present invention provides a cost effective biotechnological process for production of bio-fuels from isolated and characterized microalgae. The algal strains used in the present invention having higher biomass, higher lipid productivity, higher pH and temperature tolerance are selected from the group consisting of Chlorella vulgaris iOC-1, Chlorella vulgaris iOC-2, Chlorella kessleri, Botrococcus bruni, Dunaliella salina and Nannochloris oculat or a combination thereof having 95-100% similarity with 18s ribosomal nucleic acids nucleotide sequences (rDNA) given for Seq. ID I, Seq. ID 2, Seq. ID 3, Seq. ID 4, Seq. ID 5 and Seq. ID 6. The present process of bio-fuel production comprises the steps of producing lipid from green algae in bioreactors by various novel steps and extracting oil from dried algal cells and ultimately producing biodiesel by transesterification of the said extracted oil.

Abstract translation: 本发明提供了用于从分离和表征的微藻生产生物燃料的成本有效的生物技术方法。 具有较高生物量，较高脂质生产力，较高pH和温度耐受性的本发明中使用的藻类菌株选自小球藻iOC-1，寻常型小球藻iOC-2，小球藻，布鲁氏菌，杜氏盐藻和Nannochloris 眼睛或其组合与具有与Seq给出的18s核糖体核酸核苷酸序列（rDNA）具有95-100％相似性。 ID I，Seq。 ID 2，Seq。 ID 3，Seq ID 4，Seq。 ID 5和Seq。 本发明的生物燃料生产方法包括以下步骤：通过各种新的步骤从生物反应器中的绿藻生产脂质，并从干藻细胞中提取油，最终通过所述提取的油的酯交换生产生物柴油。