公开(公告)号：US10329350B2

公开(公告)日：2019-06-25

申请号：US13761569

申请日：2013-02-07

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Min-Yuan Chou ,  Chuan-Chuan Huang ,  Hsiu-Chuan Li ,  Ya-Ping Lai

IPC: C07K16/28 ,  C07K16/46 ,  A61K39/00

Abstract: The present invention relates to a method for producing multivalent Fab fragments. In particular, the invention relates to a method for the generation of trimeric Fab fragments by co-expression of a gene construct comprising a heavy chain portion of a Fab fragment and an in-frame fused collagen-like peptide, and a gene construct consisting of a light chain portion of an IgG in mammalian cells. Uses of molecules generated using the method of the invention are also described.