公开(公告)号：US20240043797A1

公开(公告)日：2024-02-08

申请号：US18267230

申请日：2021-12-10

Applicant: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, CHOSUN UNIVERSITY ,  KONKUK UNIVERSITY INDUSTRIAL COOPERATION CORP

Inventor： Tae-Hyoung KIM ,  Jung Hee PARK ,  Ji-Hye HAN ,  Seung-Hyun MYUNG ,  Ssang-Goo CHO ,  Kyung Min LIM ,  Yoon Joo LEE

IPC: C12N5/073 ,  A61P17/02 ,  A23L33/10 ,  A61P29/00

CPC classification number: C12N5/0605 ,  A61P17/02 ,  A23L33/10 ,  A61P29/00 ,  C12N2500/34 ,  C12N2500/30 ,  C12N2501/734

Abstract: The present disclosure relates to a method for mass production of extracellular vesicles by using a noxa protein-derived peptide and mesenchymal stem cells and, more specifically, to a method for mass production of extracellular vesicles having wound healing and immunomodulatory effects, wherein mesenchymal stem cells are cultured in a medium composition containing a noxa protein-derived peptide, glucose, sucrose, and 3-(N-morpholino)propanesulfonic acid [MOPS], whereby the extracellular vesicles can be obtained at high yield with high purity.

公开(公告)号：US20220304931A1

公开(公告)日：2022-09-29

申请号：US17424232

申请日：2021-06-03

Applicant: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, CHOSUN UNIVERSITY

Inventor： Tae-Hyoung KIM ,  Jung Hee PARK ,  Ji Hye HAN ,  Seung-Hyun MYUNG

IPC: A61K9/127 ,  A61K38/17 ,  A61K48/00 ,  A61K31/704 ,  A61K31/473 ,  C12N5/09 ,  C12N15/88

Abstract: The present invention relates to a method for producing extracellular vesicles (EV) in large quantities by using a peptide derived from Noxa protein that plays a key role in apoptosis and derivatives thereof, and the use of the peptide for promoting extracellular vesicle production and the method for producing extracellular vesicles by using the same of the present invention can efficiently and uniformly mass-produce extracellular vesicles, and can produce extracellular vesicles which load drugs passing poorly through cellular membranes as well as recombinant proteins or plasmid DNA.

公开(公告)号：US20200239521A1

公开(公告)日：2020-07-30

申请号：US16774423

申请日：2020-01-28

Applicant: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, CHOSUN UNIVERSITY

Inventor： Tae-Hyoung KIM ,  Junghee PARK ,  Ji-Hye HAN ,  Seung-Hyun MYUNG

IPC: C07K7/08 ,  A61P35/00 ,  A61K47/62 ,  A61K38/00

Abstract: Disclosed herein is a cell death-inducing peptide that rapidly acts. The peptide is derived from Noxa protein and comprises 16 amino acid residues including MTD. The peptide is designated extended MTD (eMTD) because it contains the 10-mer MTD. eMTD rapidly exhibits potent necrotic cell death in various cell lines and, as such, can be applied to the treatment of various diseases including cancer when used in conjugation with peptides or materials for targeting specific cells.