公开(公告)号：US20160084823A1

公开(公告)日：2016-03-24

申请号：US14785489

申请日：2014-04-17

申请人： INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE ,  UNIVERSITÉ DE ROUEN ,  CENTRE HOSPITALIER UNIVERSITAIRE DE ROUEN

发明人： Jean-Michel FLAMAN ,  Thierry FREBOURG ,  Gaelle BOUGEARD ,  Yasmine ZERDOUMI

IPC分类号： G01N33/50

CPC分类号： G01N33/5023 ,  G01N33/5014

摘要： The present invention relates to methods and kits for testing the genotoxicity of an agent. In particular, the present invention provides a global assay for the measurement of genotoxic stress based on p53 target gene induction. In particular, the present invention relates to a method for testing the genotoxicity of an agent comprising the steps consisting of i) providing a population of test cells, ii) exposing the populations of test cells with the agent to be tested, iii) determining the expression level of at least one p53 target gene selected from the group consisting of ATF3, BBC3, CABYR, C10ORF10; EMX1; FHL2, GLS2; GRHL3; HES2; IGF-BP4; KIAA0284; PODXL1; RRAD; TP53I3, and XPC in the population of test cells exposed to the agent to be tested iv) comparing the expression level determined at step iii) with the expression level determined when the population of test cells was not exposed to the agent to be tested, and v) concluding that the agent is genotoxic when the expression level determined at step iii) is higher than the level determined when the population of test cells was not exposed to the agent to be tested.

摘要翻译： 本发明涉及用于测试药剂基因毒性的方法和试剂盒。 特别地，本发明提供了基于p53靶基因诱导测量基因毒性应激的全局测定。 特别地，本发明涉及一种用于测试药剂的基因毒性的方法，包括以下步骤：i）提供一组测试细胞，ii）将测试细胞群暴露于待测试的试剂中，iii）确定 至少一种选自ATF3，BBC3，CABYR，C10ORF10的p53靶基因的表达水平; EMX1; FHL2，GLS2; GRHL3; HES2; IGF-BP4; KIAA0284; PODXL1; RRAD; TP53I3和XPC在暴露于待测试试剂的测试细胞群体中iv）将步骤iii）中确定的表达水平与当测试细胞群体未暴露于待测试试剂时测定的表达水平进行比较，以及 v）得出结论，当在步骤iii）中确定的表达水平高于当测试细胞群体未暴露于待测试试剂时确定的水平时，该试剂是基因毒性。