公开(公告)号：US12110544B2

公开(公告)日：2024-10-08

申请号：US17058964

申请日：2018-07-13

Applicant: INSTITUTE OF ANIMAL SCIENCES, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

Inventor： Jiaqi Wang ,  Shengguo Zhao ,  Nan Zheng ,  Xu Zhou ,  Songli Li

IPC: C12Q1/686 ,  C12N15/10 ,  C12Q1/6806

CPC classification number: C12Q1/686 ,  C12N15/1003 ,  C12Q1/6806

Abstract: In a method for extracting microbial DNA from milk, the extracted DNA can be directly used for PCR amplification. The method includes the following steps: treating milk with TE solution, fully disrupting microbial cells by bead milling, immobilizing proteins in the middle layer between the aqueous and organic phases with high-vacuum silicone grease so as to prevent impurities in the middle layer from entering the aqueous phase, etc. The extracted DNA has a high degree of intactness, high concentration and purity.