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1.
公开(公告)号:US20130252243A1
公开(公告)日:2013-09-26
申请号:US13724825
申请日:2012-12-21
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6876 , C12N15/111 , C12N2310/11 , C12N2310/14 , C12N2320/10 , Y10T436/143333
摘要: Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2.
摘要翻译: 本文提供了确定RNAi剂效力的方法。 这样的方法包括但不限于测量RNAi剂与Ago2的结合或在这种RNAi剂存在下测量Ago2活性的基于细胞和无细胞的测定。 还提供了通过评估其与其他RNAi试剂(包括对照RNAi试剂)竞争与Ago2结合和/或激活的能力来测定RNAi试剂的效力的测定法。