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公开(公告)号：US20190048386A1
公开(公告)日：2019-02-14
申请号：US16145196
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/02 , C12N9/04 , C12Q1/32 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190177761A1
公开(公告)日：2019-06-13
申请号：US15927976
申请日：2018-03-21
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190177763A1
公开(公告)日：2019-06-13
申请号：US15928027
申请日：2018-03-21
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190017087A1
公开(公告)日：2019-01-17
申请号：US16145165
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron accepter and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190062798A1
公开(公告)日：2019-02-28
申请号：US16145178
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12Q1/32 , C12N9/02
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190040442A1
公开(公告)日：2019-02-07
申请号：US16145184
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/02 , C12N9/04 , C12Q1/32 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190040437A1
公开(公告)日：2019-02-07
申请号：US15927949
申请日：2018-03-21
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190017085A1
公开(公告)日：2019-01-17
申请号：US16145132
申请日：2018-09-27
发明人： Hironori OMURA , Hirokazu SANADA , Takako Yada , Ayaka Atsumi , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190177762A1
公开(公告)日：2019-06-13
申请号：US15928012
申请日：2018-03-21
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190040441A1
公开(公告)日：2019-02-07
申请号：US16145174
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Testunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/02 , C12N9/04 , C12Q1/32 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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