公开(公告)号：US11853396B2

公开(公告)日：2023-12-26

申请号：US18154603

申请日：2023-01-13

申请人： Illumina, Inc.

发明人： Eric Jon Ojard ,  Abde Ali Hunaid Kagalwalla ,  Rami Mehio ,  Nitin Udpa ,  Gavin Derek Parnaby ,  John S Vieceli

IPC分类号： G06N3/08 ,  G06F18/2411 ,  G06T7/187 ,  G06V10/50 ,  G06F18/2135

CPC分类号： G06F18/2411 ,  G06F18/21355 ,  G06T7/187 ,  G06V10/507

摘要： The technology disclosed corrects inter-cluster intensity profile variation for improved base calling on a cluster-by-cluster basis. The technology disclosed accesses current intensity data and historic intensity data of a target cluster, where the current intensity data is for a current sequencing cycle and the historic intensity data is for one or more preceding sequencing cycles. A first accumulated intensity correction parameter is determined by accumulating distribution intensities measured for the target cluster at the current and preceding sequencing cycles. A second accumulated intensity correction parameter is determined by accumulating intensity errors measured for the target cluster at the current and preceding sequencing cycles. Based on the first and second accumulated intensity correction parameters, next intensity data for a next sequencing cycle is corrected to generate corrected next intensity data, which is used to base call the target cluster at the next sequencing cycle.