INTER-CLUSTER INTENSITY VARIATION CORRECTION AND BASE CALLING

    公开(公告)号:US20240220584A1

    公开(公告)日:2024-07-04

    申请号:US18501904

    申请日:2023-11-03

    Applicant: Illumina, Inc.

    CPC classification number: G06F18/2411 G06F18/21355 G06T7/187 G06V10/507

    Abstract: The technology disclosed corrects inter-cluster intensity profile variation for improved base calling on a cluster-by-cluster basis. The technology disclosed accesses current intensity data and historic intensity data of a target cluster, where the current intensity data is for a current sequencing cycle and the historic intensity data is for one or more preceding sequencing cycles. A first accumulated intensity correction parameter is determined by accumulating distribution intensities measured for the target cluster at the current and preceding sequencing cycles. A second accumulated intensity correction parameter is determined by accumulating intensity errors measured for the target cluster at the current and preceding sequencing cycles. Based on the first and second accumulated intensity correction parameters, next intensity data for a next sequencing cycle is corrected to generate corrected next intensity data, which is used to base call the target cluster at the next sequencing cycle.

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