专利检索 ap:("Immaculada Margarit y Ros" OR "Susanna Campagnoli" OR "Roberto Gianna" OR "Salvatore Toma" OR "Guido Grandi") AND inv:"Immaculada Margarit y Ros" 第 1 页

1.

发明授权
Thermostable mutants of neutral protease and means for their preparation 失效
标题翻译：中性蛋白酶的热稳定突变体及其制备方法

公开(公告)号：US5252478A

公开(公告)日：1993-10-12

申请号：US658908

申请日：1991-02-22

申请人： Immaculada Margarit y Ros , Susanna Campagnoli , Roberto Gianna , Salvatore Toma , Guido Grandi

发明人： Immaculada Margarit y Ros , Susanna Campagnoli , Roberto Gianna , Salvatore Toma , Guido Grandi

IPC分类号： C12N1/21 , C12N9/50 , C12N9/56 , C12N15/57 , C12N15/00 , C12N15/75

CPC分类号： C12N9/54 , C12N9/50

摘要： The mutants of neutral protease (NP) described can retain their enzymatic activities at temperatures at which wild-type neutral protease becomes inactive, the mutants being characterised in that the Gly 189 and/or Gly 147 residues of the aminoacid sequence of NP are replaced by different residues selected from the natural aminoacids.Mutagenised genes of neutral protease which code for the mutants, recombinant plasmids containing the genes, and strains of Bacillus transformed by the plasmids are also described. The mutants of neutral protease having the characteristics described above are particularly useful in the food sector.