公开(公告)号：US10208358B2

公开(公告)日：2019-02-19

申请号：US15304019

申请日：2015-04-14

Applicant: Indiana University Research and Technology Corporation

Inventor： Adam Zlotnick ,  Stella Vieweger

IPC: C12N7/04 ,  A61K39/12 ,  A61K39/17 ,  C12N7/00 ,  C12Q1/68 ,  C12Q1/70 ,  C12Q1/6818

Abstract: Virus capsids protect the viral genome and play roles in its delivery and intracellular transport, making them an attractive target for antiviral therapeutics. The difficulty in targeting capsid assembly is to identify molecules that interfere with the weak protein-protein interactions that drive the reaction. We have developed an in vitro assay for capsid assembly that works on a range of viruses at biologically relevant protein concentrations to facilitate screening large libraries of chemicals for lead compounds.

公开(公告)号：US20170029907A1

公开(公告)日：2017-02-02

申请号：US15304019

申请日：2015-04-14

Applicant: Indiana University Research and Technology Corporation

Inventor： Adam Zlotnick ,  Stella Vieweger

IPC: C12Q1/70 ,  C12Q1/68

CPC classification number: C12Q1/703 ,  C12Q1/6818 ,  C12Q1/70 ,  C12Q2600/136 ,  Y02A50/53

Abstract: Virus capsids protect the viral genome and play roles in its delivery and intracellular transport, making them an attractive target for antiviral therapeutics. The difficulty in targetting capsid assembly is to identify molecules that interfere with the weak protein-protein interactions that drive the reaction. We have developed an in vitro assay for capsid assembly that works on a range of viruses at biologically relevant protein concentrations to facilitate screening large libraries of chemicals for lead compounds.

Abstract translation: 病毒衣壳保护病毒基因组并在其递送和细胞内运输中发挥作用，使其成为抗病毒治疗的有吸引力的靶标。 靶向衣壳装配的困难是确定干扰促进反应的弱蛋白质 - 蛋白质相互作用的分子。 我们开发了一种用于衣壳组装的体外测定法，其用于生物相关蛋白浓度的一系列病毒，以便筛选铅化合物的大型化学物质文库。