公开(公告)号：US07855072B2

公开(公告)日：2010-12-21

申请号：US11506143

申请日：2006-08-16

申请人： Inhwan Hwang ,  Dae Heon Kim ,  Yong Jik Lee

发明人： Inhwan Hwang ,  Dae Heon Kim ,  Yong Jik Lee

IPC分类号： C07H17/00 ,  C07K14/00

CPC分类号： C07K19/00 ,  C12Q1/37 ,  G01N2500/04

摘要： Disclosed is a system for detecting a protease inside a cell. In one embodiment, the system includes a chimeric protein that comprises as covalently linked components: 1) at least one optionally masked signal protein; 2) at least one protease-specific cleavage site; and 3) at least one detectable amino acid sequence. The invention has a wide spectrum of applications including use in the detection of novel protease inhibitors inside cells and tissue.

摘要翻译： 公开了一种用于检测细胞内的蛋白酶的系统。 在一个实施方案中，该系统包括嵌合蛋白，其包含作为共价连接的组分：1）至少一种任选地掩蔽的信号蛋白; 2）至少一个蛋白酶特异性切割位点; 和3）至少一个可检测的氨基酸序列。 本发明具有广泛的应用，包括用于检测细胞和组织内的新型蛋白酶抑制剂。

公开(公告)号：US20110294697A1

公开(公告)日：2011-12-01

申请号：US12962038

申请日：2010-12-07

申请人： Inhwan Hwang ,  Dae Heon Kim ,  Yong Jik Lee

发明人： Inhwan Hwang ,  Dae Heon Kim ,  Yong Jik Lee

IPC分类号： C40B30/06 ,  C12N15/63 ,  C12N9/12 ,  C12N9/14 ,  C12N9/02 ,  C12Q1/70 ,  C12N5/10 ,  C12N1/19 ,  C12N1/15 ,  C12N1/21 ,  C07K19/00 ,  C07H21/00 ,  C12Q1/68 ,  C12N9/04

CPC分类号： C07K19/00 ,  C12Q1/37 ,  G01N2500/04

摘要： Disclosed is a system for detecting a protease inside a cell. In one embodiment, the system includes a chimeric protein that comprises as covalently linked components: 1) at least one optionally masked signal protein; 2) at least one protease-specific cleavage site; and 3) at least one detectable amino acid sequence. The invention has a wide spectrum of applications including use in the detection of novel protease inhibitors inside cells and tissue.

摘要翻译： 公开了一种用于检测细胞内的蛋白酶的系统。 在一个实施方案中，该系统包括嵌合蛋白，其包含作为共价连接的组分：1）至少一种任选地掩蔽的信号蛋白; 2）至少一个蛋白酶特异性切割位点; 和3）至少一个可检测的氨基酸序列。 本发明具有广泛的应用，包括用于检测细胞和组织内的新型蛋白酶抑制剂。

公开(公告)号：US07109293B2

公开(公告)日：2006-09-19

申请号：US10214932

申请日：2002-08-08

申请人： Inhwan Hwang ,  Dae Heon Kim ,  Yong Jik Lee

发明人： Inhwan Hwang ,  Dae Heon Kim ,  Yong Jik Lee

IPC分类号： C07K14/00

CPC分类号： C07K19/00 ,  C12Q1/37 ,  G01N2500/04

摘要： Disclosed is a system for detecting a protease inside a cell. In one embodiment, the system includes a chimeric protein that comprises as covalently linked components: 1) at least one optionally masked signal protein; 2) at least one protease-specific cleavage site; and 3) at least one detectable amino acid sequence. The invention has a wide spectrum of applications including use in the detection of novel protease inhibitors inside cells and tissue.