公开(公告)号：US20150176026A1

公开(公告)日：2015-06-25

申请号：US14384687

申请日：2012-03-13

申请人： Inje University Industry-Academic Cooperation Foundation

发明人： Yeon Soo Kim ,  Moon Kyung Kang ,  A Young Song

IPC分类号： C12N15/86 ,  A61K38/16 ,  A61K38/45 ,  A61K35/76

CPC分类号： C12N15/86 ,  A61K35/76 ,  A61K38/162 ,  A61K38/45 ,  A61K48/00 ,  C12N2740/10032 ,  C12N2740/10043 ,  C12N2740/13045 ,  C12N2740/13052 ,  C12N2800/40 ,  C12N2800/50 ,  C12Y207/01021

摘要： The present invention provides a vector system in which a MuLV-Gag gene, a MuLV-Pol gene, and a GaLV-Env gene are expressed in two separate vectors. The vector system is capable of inserting a therapeutic gene to these two separate vectors, and in this raged, the size of an inserted gene is not limited and a variety of foreign therapeutic genes may be inserted to the vectors. Accordingly, the foreign therapeutic gene may be delivered in a safe and efficient manner to desired tissue of cells of aberrant proliferation. Therefore, the vector system is applicable in a composition for delivering a gene targeting the aberrantly dividing cells of aberrant proliferation, wherein the composition includes a retrovirus produced by cell line transfection. The vector system is also applicable in a composition for preventing or treating a disease caused by cells of aberrant proliferation of, such as cancer cells.

摘要翻译： 本发明提供了在两个分开的载体中表达MuLV-Gag基因，MuLV-Pol基因和GaLV-Env基因的载体系统。 载体系统能够将治疗基因插入到这两个单独的载体中，并且在这种情况下，插入的基因的大小不受限制，并且可以将多种外来治疗基因插入到载体中。 因此，外来治疗基因可以以安全有效的方式递送到期望的异常增殖细胞组织。 因此，载体系统可用于递送针对异常增殖异常分化细胞的基因的组合物，其中该组合物包含通过细胞系转染产生的逆转录病毒。 载体系统也可用于预防或治疗由诸如癌细胞异常增殖的细胞引起的疾病的组合物。